The application of direct-injection mass spectrometric (DI-MS) techniques, like Proton Transfer Reaction Time of Flight Mass Spectrometry (PTR-ToF-MS) has been suggested as a reliable phenotyping tool for fruit volatilome assessment in both genetic and quality-related studies. In this study the complexity of raspberry aroma was investigated by a comprehensive untargeted VOC analysis, done by combining SPME-GC-MS and PTR-ToF-MS assessments with multi-block discriminant analysis using the DIABLO mixOmics framework. The aim was to acquire an exhaustive characterization of the raspberry volatilome according to different fruit ripening stages (pink, ripe, and overripe) and genetic variances (50 accessions), as well as to investigate the potential of PTR-ToF-MS as a rapid and high throughput VOC phenotyping tool to address issues related to raspberry fruit quality. Results of this study demonstrated the complementarity between SPME-GC-MS and PTR-ToF-MS techniques to evaluate the raspberry aroma composition. PTR-ToF-MS generates reliable raspberry VOC fingerprints mainly due to a reduced compound fragmentation and precise content estimation. In addition, the high collinearity between isomers of monoterpenes and norisoprenoids, discovered by GC analysis, reduces the main analytic limitation of PTR-ToF-MS of not being able to separate isomeric molecules. The high similarity between the VOC matrices obtained by applying PTR-ToF-MS and SPME-GC-MS confirmed the possibility of using PTR-ToF-MS as a reliable high throughput phenotyping tool for raspberry volatiolome assessment. In addition, results provided by the germplasm collection investigation enabled to distinguish the best performing accessions, based on VOCs composition, to be used as superior parental lines for future breeding programs

Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques / Farneti, Brian; Khomenko, Iuliia; Ajelli, Matteo; Wells, Karen Elizabeth; Betta, Emanuela; Aprea, Eugenio; Giongo, Lara; Biasioli, Franco. - In: FRONTIERS IN MOLECULAR BIOSCIENCES. - ISSN 2296-889X. - 10:(2023), pp. 1-12. [10.3389/fmolb.2023.1155564]

Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques

Wells, Karen Elizabeth;Betta, Emanuela;Aprea, Eugenio;
2023-01-01

Abstract

The application of direct-injection mass spectrometric (DI-MS) techniques, like Proton Transfer Reaction Time of Flight Mass Spectrometry (PTR-ToF-MS) has been suggested as a reliable phenotyping tool for fruit volatilome assessment in both genetic and quality-related studies. In this study the complexity of raspberry aroma was investigated by a comprehensive untargeted VOC analysis, done by combining SPME-GC-MS and PTR-ToF-MS assessments with multi-block discriminant analysis using the DIABLO mixOmics framework. The aim was to acquire an exhaustive characterization of the raspberry volatilome according to different fruit ripening stages (pink, ripe, and overripe) and genetic variances (50 accessions), as well as to investigate the potential of PTR-ToF-MS as a rapid and high throughput VOC phenotyping tool to address issues related to raspberry fruit quality. Results of this study demonstrated the complementarity between SPME-GC-MS and PTR-ToF-MS techniques to evaluate the raspberry aroma composition. PTR-ToF-MS generates reliable raspberry VOC fingerprints mainly due to a reduced compound fragmentation and precise content estimation. In addition, the high collinearity between isomers of monoterpenes and norisoprenoids, discovered by GC analysis, reduces the main analytic limitation of PTR-ToF-MS of not being able to separate isomeric molecules. The high similarity between the VOC matrices obtained by applying PTR-ToF-MS and SPME-GC-MS confirmed the possibility of using PTR-ToF-MS as a reliable high throughput phenotyping tool for raspberry volatiolome assessment. In addition, results provided by the germplasm collection investigation enabled to distinguish the best performing accessions, based on VOCs composition, to be used as superior parental lines for future breeding programs
2023
Farneti, Brian; Khomenko, Iuliia; Ajelli, Matteo; Wells, Karen Elizabeth; Betta, Emanuela; Aprea, Eugenio; Giongo, Lara; Biasioli, Franco
Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques / Farneti, Brian; Khomenko, Iuliia; Ajelli, Matteo; Wells, Karen Elizabeth; Betta, Emanuela; Aprea, Eugenio; Giongo, Lara; Biasioli, Franco. - In: FRONTIERS IN MOLECULAR BIOSCIENCES. - ISSN 2296-889X. - 10:(2023), pp. 1-12. [10.3389/fmolb.2023.1155564]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/376430
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