Soil microbiome comprises numerous microbial species that continuously interact with each other. Among the modes of diverse interactions, cell–cell killing may play a key role in shaping the microbiome composition. Bacteria deploy various secretion systems to fend off other microorganisms and Type IV Secretion System (T4SS) in pathogenic bacteria was shown to function as a contact-dependent, inter-bacterial killing system only recently. The present study investigated the role played by T4SS in the killing behaviour of the soilborne biocontrol bacterium Lysobacter enzymogenes OH11. Results showed that L. enzymogenes OH11 genome encompasses genes encoding all the components of T4SS and effectors potentially involved in inter-bacterial killing system. Generation of knock-out mutants revealed that L. enzymogenes OH11 uses T4SS as the main contact-dependent weapon against other soilborne bacteria. The T4SS-mediated killing behaviour of L. enzymogenes OH11 decreased the antibacterial and antifungal activity of two Pseudomonas spp. but at the same time, protected carrot from infection by Pectobacterium carotovorum. Overall, this study showed for the first time the involvement of T4SS in the killing behaviour of L. enzymogenes and its impact on the multiple interactions occurring in the soil microbiome.

Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system / Shen, X.; Wang, B.; Yang, N.; Zhang, L.; Shen, D.; Wu, H.; Dong, Y.; Niu, B.; Chou, S. -H.; Puopolo, G.; Fan, J.; Qian, G.. - In: ENVIRONMENTAL MICROBIOLOGY. - ISSN 1462-2912. - 2021/23:8(2021), pp. 4673-4688. [10.1111/1462-2920.15662]

Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system

Wang B.;Yang N.;Wu H.;Dong Y.;Puopolo G.;
2021-01-01

Abstract

Soil microbiome comprises numerous microbial species that continuously interact with each other. Among the modes of diverse interactions, cell–cell killing may play a key role in shaping the microbiome composition. Bacteria deploy various secretion systems to fend off other microorganisms and Type IV Secretion System (T4SS) in pathogenic bacteria was shown to function as a contact-dependent, inter-bacterial killing system only recently. The present study investigated the role played by T4SS in the killing behaviour of the soilborne biocontrol bacterium Lysobacter enzymogenes OH11. Results showed that L. enzymogenes OH11 genome encompasses genes encoding all the components of T4SS and effectors potentially involved in inter-bacterial killing system. Generation of knock-out mutants revealed that L. enzymogenes OH11 uses T4SS as the main contact-dependent weapon against other soilborne bacteria. The T4SS-mediated killing behaviour of L. enzymogenes OH11 decreased the antibacterial and antifungal activity of two Pseudomonas spp. but at the same time, protected carrot from infection by Pectobacterium carotovorum. Overall, this study showed for the first time the involvement of T4SS in the killing behaviour of L. enzymogenes and its impact on the multiple interactions occurring in the soil microbiome.
2021
8
Shen, X.; Wang, B.; Yang, N.; Zhang, L.; Shen, D.; Wu, H.; Dong, Y.; Niu, B.; Chou, S. -H.; Puopolo, G.; Fan, J.; Qian, G.
Lysobacter enzymogenes antagonizes soilborne bacteria using the type IV secretion system / Shen, X.; Wang, B.; Yang, N.; Zhang, L.; Shen, D.; Wu, H.; Dong, Y.; Niu, B.; Chou, S. -H.; Puopolo, G.; Fan, J.; Qian, G.. - In: ENVIRONMENTAL MICROBIOLOGY. - ISSN 1462-2912. - 2021/23:8(2021), pp. 4673-4688. [10.1111/1462-2920.15662]
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