Background: The coronavirus disease 2019 (COVID-19) outbreak, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been declared a global pandemic. It is well-established that SARS-CoV-2 infection can lead to dysregulated immune responses. Arginase-1 (Arg1), which has a pivotal role in immune cells, can be expressed in most of the myeloid cells, e.g., neutrophils and macrophages. Arg1 has been associated with the suppression of antiviral immune responses. Methods: Whole blood was taken from 21 COVID-19 patients and 21 healthy individuals, and after RNA extraction and complementary DNA (cDNA) synthesis, gene expression of Arg1 was measured by real-time PCR. Results: The qPCR results showed that the expression of Arg1 was significantly increased in COVID-19 patients compared to healthy individuals (p < 0.01). The relative expression analysis demonstrated there were approximately 2.3 times increased Arg1 expression in the whole blood of COVID-19 patients. Furthermore, the receiver operating characteristic (ROC) analysis showed a considerable diagnostic value for Arg1 expression in COVID-19 (p = 0.0002 and AUC = 0.8401). Conclusion: Arg1 might be a promising marker in the pathogenesis of the disease, and it could be a valuable diagnostic tool.

Arginase 1 (Arg1) as an Up-Regulated Gene in COVID-19 Patients: A Promising Marker in COVID-19 Immunopathy / Derakhshani, Afshin; Hemmat, Nima; Asadzadeh, Zahra; Ghaseminia, Moslem; Shadbad, Mahdi Abdoli; Jadideslam, Golamreza; Silvestris, Nicola; Racanelli, Vito; Baradaran, Behzad. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 10:5(2021), pp. 10511-10519. [10.3390/jcm10051051]

Arginase 1 (Arg1) as an Up-Regulated Gene in COVID-19 Patients: A Promising Marker in COVID-19 Immunopathy

Racanelli, Vito;
2021-01-01

Abstract

Background: The coronavirus disease 2019 (COVID-19) outbreak, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been declared a global pandemic. It is well-established that SARS-CoV-2 infection can lead to dysregulated immune responses. Arginase-1 (Arg1), which has a pivotal role in immune cells, can be expressed in most of the myeloid cells, e.g., neutrophils and macrophages. Arg1 has been associated with the suppression of antiviral immune responses. Methods: Whole blood was taken from 21 COVID-19 patients and 21 healthy individuals, and after RNA extraction and complementary DNA (cDNA) synthesis, gene expression of Arg1 was measured by real-time PCR. Results: The qPCR results showed that the expression of Arg1 was significantly increased in COVID-19 patients compared to healthy individuals (p < 0.01). The relative expression analysis demonstrated there were approximately 2.3 times increased Arg1 expression in the whole blood of COVID-19 patients. Furthermore, the receiver operating characteristic (ROC) analysis showed a considerable diagnostic value for Arg1 expression in COVID-19 (p = 0.0002 and AUC = 0.8401). Conclusion: Arg1 might be a promising marker in the pathogenesis of the disease, and it could be a valuable diagnostic tool.
2021
5
Derakhshani, Afshin; Hemmat, Nima; Asadzadeh, Zahra; Ghaseminia, Moslem; Shadbad, Mahdi Abdoli; Jadideslam, Golamreza; Silvestris, Nicola; Racanelli, ...espandi
Arginase 1 (Arg1) as an Up-Regulated Gene in COVID-19 Patients: A Promising Marker in COVID-19 Immunopathy / Derakhshani, Afshin; Hemmat, Nima; Asadzadeh, Zahra; Ghaseminia, Moslem; Shadbad, Mahdi Abdoli; Jadideslam, Golamreza; Silvestris, Nicola; Racanelli, Vito; Baradaran, Behzad. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 10:5(2021), pp. 10511-10519. [10.3390/jcm10051051]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/386982
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