Signal Processing Methods for Reliable Extraction of Neural Responses in Developmental EEG

Studying newborns in the first days of life prior to experiencing the world provides remarkable insights into the neurocognitive predispositions that humans are endowed with. First, it helps us to improve our current knowledge of the development of a typical brain. Secondly, it potentially opens new pathways for earlier diagnosis of several developmental neurocognitive disorders such as Autism Spectrum Disorder (ASD). While most studies investigating early cognition in the literature are purely behavioural, recently there has been an increasing number of neuroimaging studies in newborns and infants. Electroencephalography (EEG) is one of the most optimal neuroimaging technique to investigate neurocognitive functions in human newborns because it is non-invasive and quick and easy to mount on the head. Since EEG offers a versatile design with custom number of channels/electrodes, an ergonomic wearable solution could help study newborns outside clinical settings such as their homes. Compared to adult EEG, newborn EEG data are different in two main aspects: 1) In experimental designs investigating stimulus-related neural responses, collected data is extremely short in length due to the reduced attentional span of newborns; 2) Data is heavily contaminated with noise due to their uncontrollable movement artifacts. Since EEG processing methods for adults are not adapted to very short data length and usually deal with well-defined, stereotyped artifacts, they are unsuitable for newborn EEG. As a result, researchers manually clean the data, which is a subjective and time-consuming task. This thesis work is specifically dedicated to developing (semi-) automated novel signal processing methods for noise removal and for extracting reliable neural responses specific to this population. The solutions are proposed for both high-density EEG for traditional lab-based research and wearable EEG for clinical applications. To this end, this thesis, first, presents novel signal processing methods applied to newborn EEG: 1) Local Outlier Factor (LOF) for detecting and removing bad/noisy channels; 2) Artifacts Subspace Reconstruction (ASR) for detecting and removing or correcting bad/noisy segments. Then, based on these algorithms and other preprocessing functionalities, a robust preprocessing pipeline, Newborn EEG Artifact Removal (NEAR), is proposed. Notably, this is the first time LOF is explored for EEG bad channel detection, despite being a popular outlier detection technique in other kinds of data such as Electrocardiogram (ECG). Even if ASR is already an established artifact real algorithm originally developed for mobile adult EEG, this thesis explores the possibility of adapting ASR for short newborn EEG data, which is the first of its kind. NEAR is validated on simulated, real newborn, and infant EEG datasets. We used the SEREEGA toolbox to simulate neurologically plausible synthetic data and contaminated a certain number of channels and segments with artifacts commonly manifested in developmental EEG. We used newborn EEG data (n = 10, age range: 1 and 4 days) recorded in our lab based on a frequency-tagging paradigm. The chosen paradigm consists of visual stimuli to investigate the cortical bases of facelike pattern processing, and the results were published in 2019. To test NEAR performance on an older population with an event-related design (ERP) and with data recorded in another lab, we also evaluated NEAR on infant EEG data recorded on 9-months-old infants (n = 14) with an ERP paradigm. The experimental paradigm for these datasets consists of auditory stimulus to investigate the electrophysiological evidence for understanding maternal speech, and the results were published in 2012. Since authors of these independent studies employed manual artifact removal, the obtained neural responses serve as ground truth for validating NEAR’s artifact removal performance. For comparative evaluation, we considered the performance of two state-of-the-art pipelines designed for older infants. Results show that NEAR is successful in recovering the neural responses (specific to the EEG paradigm and the stimuli) compared to the other pipelines. In sum, this thesis presents a set of methods for artifact removal and extraction of stimulus-related neural responses specifically adapted to newborn and infant EEG data that will hopefully contribute to strengthening the reliability and reproducibility of developmental cognitive neuroscience studies, both in research laboratories and in clinical applications.