When Bacillus subtilis strain ATCC 21951, a transketolase-deficient D-ribose-producing mutant, was grown on D-glucose plus a second substrate which is metabolized via the oxidative pentose phosphate cycle (D-gluconic acid, D-xylose, L-arabinose or D-xylitol), D-glucose did not catabolite repress metabolism of the second carbon source. The D-ribose yield obtained with the simultaneously converted carbon substrates, significantly exceeded that when only D-glucose was used. In addition, the concentration of glycolytic by-products and the fermentation time significantly decreased. Based on these findings, a fermentation process was developed with B. subtilis strain ATCC 21951 in which D-glucose (100 g L -1) and D-gluconic acid (50 g L -1) were converted into 45 g L -1 of D-ribose and 7.5 g L -1 of acetoin, A second process, based on D-glucose and D-xylose (100 g L -1 each), yielded 60 g L -1 of D-ribose and 4 g L -1 of acetoin plus 2,3-butanediol. Both mixed carbon source fermentations provide excellent alternatives to the less efficient D-glucose-based processes used so far.

D-Glucose does not catabolite repress a transketolase-deficient D-ribose-producing Bacillus subtilis mutant strain / De Wulf, P.; Soetaert, W.; Schwengers, D.; Vandamme, E. J.. - In: JOURNAL OF INDUSTRIAL MICROBIOLOGY. - ISSN 0169-4146. - ELETTRONICO. - 1996, vol.17:2(1996), pp. 104-109.

D-Glucose does not catabolite repress a transketolase-deficient D-ribose-producing Bacillus subtilis mutant strain

De Wulf P.;
1996-01-01

Abstract

When Bacillus subtilis strain ATCC 21951, a transketolase-deficient D-ribose-producing mutant, was grown on D-glucose plus a second substrate which is metabolized via the oxidative pentose phosphate cycle (D-gluconic acid, D-xylose, L-arabinose or D-xylitol), D-glucose did not catabolite repress metabolism of the second carbon source. The D-ribose yield obtained with the simultaneously converted carbon substrates, significantly exceeded that when only D-glucose was used. In addition, the concentration of glycolytic by-products and the fermentation time significantly decreased. Based on these findings, a fermentation process was developed with B. subtilis strain ATCC 21951 in which D-glucose (100 g L -1) and D-gluconic acid (50 g L -1) were converted into 45 g L -1 of D-ribose and 7.5 g L -1 of acetoin, A second process, based on D-glucose and D-xylose (100 g L -1 each), yielded 60 g L -1 of D-ribose and 4 g L -1 of acetoin plus 2,3-butanediol. Both mixed carbon source fermentations provide excellent alternatives to the less efficient D-glucose-based processes used so far.
1996
2
De Wulf, P.; Soetaert, W.; Schwengers, D.; Vandamme, E. J.
D-Glucose does not catabolite repress a transketolase-deficient D-ribose-producing Bacillus subtilis mutant strain / De Wulf, P.; Soetaert, W.; Schwengers, D.; Vandamme, E. J.. - In: JOURNAL OF INDUSTRIAL MICROBIOLOGY. - ISSN 0169-4146. - ELETTRONICO. - 1996, vol.17:2(1996), pp. 104-109.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/262047
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? ND
social impact