BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a progressive and fatal motor neuron disease, and protein aggregation has been proposed as a possible pathogenetic mechanism. However, the aggregate protein constituents are poorly characterized so knowledge on the role of aggregation in pathogenesis is limited. METHODOLOGY/PRINCIPAL FINDINGS: We carried out a proteomic analysis of the protein composition of the insoluble fraction, as a model of protein aggregates, from familial ALS (fALS) mouse model at different disease stages. We identified several proteins enriched in the detergent-insoluble fraction already at a preclinical stage, including intermediate filaments, chaperones and mitochondrial proteins. Aconitase, HSC70 and cyclophilin A were also significantly enriched in the insoluble fraction of spinal cords of ALS patients. Moreover, we found that the majority of proteins in mice and HSP90 in patients were tyrosine-nitrated. We therefore investigated the role of nitrative stress in aggregate formation in fALS-like murine motor neuron-neuroblastoma (NSC-34) cell lines. By inhibiting nitric oxide synthesis the amount of insoluble proteins, particularly aconitase, HSC70, cyclophilin A and SOD1 can be substantially reduced. CONCLUSION/SIGNIFICANCE: Analysis of the insoluble fractions from cellular/mouse models and human tissues revealed novel aggregation-prone proteins and suggests that nitrative stress contribute to protein aggregate formation in ALS.

Characterization of detergent-insoluble proteins in ALS indicates a causal link between nitrative stress and aggregation in pathogenesis / Basso, Manuela; Samengo, Giuseppina; Nardo, Giovanni; Massignan, Tania; D'Alessandro, Giuseppina; Tartari, Silvia; Cantoni, Lavinia; Marino, Marianna; Cheroni, Cristina; De Biasi, Silvia; Giordana, Maria Teresa; Strong, Michael J; Estevez, Alvaro G; Salmona, Mario; Bendotti, Caterina; Bonetto, Valentina. - In: PLOS ONE. - ISSN 1932-6203. - 4:12(2009), pp. e8130.1-e8130.15. [10.1371/journal.pone.0008130]

Characterization of detergent-insoluble proteins in ALS indicates a causal link between nitrative stress and aggregation in pathogenesis

Basso, Manuela;Massignan, Tania;
2009

Abstract

BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a progressive and fatal motor neuron disease, and protein aggregation has been proposed as a possible pathogenetic mechanism. However, the aggregate protein constituents are poorly characterized so knowledge on the role of aggregation in pathogenesis is limited. METHODOLOGY/PRINCIPAL FINDINGS: We carried out a proteomic analysis of the protein composition of the insoluble fraction, as a model of protein aggregates, from familial ALS (fALS) mouse model at different disease stages. We identified several proteins enriched in the detergent-insoluble fraction already at a preclinical stage, including intermediate filaments, chaperones and mitochondrial proteins. Aconitase, HSC70 and cyclophilin A were also significantly enriched in the insoluble fraction of spinal cords of ALS patients. Moreover, we found that the majority of proteins in mice and HSP90 in patients were tyrosine-nitrated. We therefore investigated the role of nitrative stress in aggregate formation in fALS-like murine motor neuron-neuroblastoma (NSC-34) cell lines. By inhibiting nitric oxide synthesis the amount of insoluble proteins, particularly aconitase, HSC70, cyclophilin A and SOD1 can be substantially reduced. CONCLUSION/SIGNIFICANCE: Analysis of the insoluble fractions from cellular/mouse models and human tissues revealed novel aggregation-prone proteins and suggests that nitrative stress contribute to protein aggregate formation in ALS.
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Basso, Manuela; Samengo, Giuseppina; Nardo, Giovanni; Massignan, Tania; D'Alessandro, Giuseppina; Tartari, Silvia; Cantoni, Lavinia; Marino, Marianna; Cheroni, Cristina; De Biasi, Silvia; Giordana, Maria Teresa; Strong, Michael J; Estevez, Alvaro G; Salmona, Mario; Bendotti, Caterina; Bonetto, Valentina
Characterization of detergent-insoluble proteins in ALS indicates a causal link between nitrative stress and aggregation in pathogenesis / Basso, Manuela; Samengo, Giuseppina; Nardo, Giovanni; Massignan, Tania; D'Alessandro, Giuseppina; Tartari, Silvia; Cantoni, Lavinia; Marino, Marianna; Cheroni, Cristina; De Biasi, Silvia; Giordana, Maria Teresa; Strong, Michael J; Estevez, Alvaro G; Salmona, Mario; Bendotti, Caterina; Bonetto, Valentina. - In: PLOS ONE. - ISSN 1932-6203. - 4:12(2009), pp. e8130.1-e8130.15. [10.1371/journal.pone.0008130]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/222767
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