Lipofectamine-based transfection was used as a method of choice to deliver the bacterial beta-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. beta-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.
Scheda prodotto non validato
I dati visualizzati non sono stati ancora sottoposti a validazione formale da parte dello Staff di IRIS, ma sono stati ugualmente trasmessi al Sito Docente Cineca (Loginmiur).
Titolo: | Non-virally mediated gene transfer into human central nervous system precursor cells. |
Autori: | Cattaneo, E; Conti, Luciano; Gritti, A; Frolichsthal, P; Govoni, S; Vescovi, A. |
Autori Unitn: | |
Titolo del periodico: | MOLECULAR BRAIN RESEARCH |
Anno di pubblicazione: | 1996 |
Numero e parte del fascicolo: | 1 |
Codice identificativo Scopus: | 2-s2.0-0030297964 |
Codice identificativo Pubmed: | 8915596 |
Codice identificativo ISI: | WOS:A1996VP33400023 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0169-328X(96)00159-3 |
Handle: | http://hdl.handle.net/11572/99162 |
Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |