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EnglishLipofectamine-based transfection was used as a method of choice to deliver the bacterial beta-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. beta-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.
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| Titolo: | Non-virally mediated gene transfer into human central nervous system precursor cells. |
| Autori Unitn: | |
| Anno di pubblicazione: | 1996 |
| Titolo del periodico: | |
| Abstract: | Lipofectamine-based transfection was used as a method of choice to deliver the bacterial beta-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. beta-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection. |
| Handle: | http://hdl.handle.net/11572/99162 |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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