Non-virally mediated gene transfer into human central nervous system precursor cells.

Lipofectamine-based transfection was used as a method of choice to deliver the bacterial beta-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. beta-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.



Titolo: Non-virally mediated gene transfer into human central nervous system precursor cells.
Autori: Cattaneo, E; Conti, Luciano; Gritti, A; Frolichsthal, P; Govoni, S; Vescovi, A.
Autori Unitn: 
Conti, Luciano
mostra contributor esterni 
Titolo del periodico: MOLECULAR BRAIN RESEARCH
Anno di pubblicazione: 1996
Numero e parte del fascicolo: 1
Codice identificativo Scopus: 2-s2.0-0030297964
Codice identificativo Pubmed: 8915596
Codice identificativo ISI: WOS:A1996VP33400023
Digital Object Identifier (DOI): http://dx.doi.org/10.1016/S0169-328X(96)00159-3
Handle: http://hdl.handle.net/11572/99162
Appare nelle tipologie:03.1 Articolo su rivista (Journal article)

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