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EnglishNS cells are a homogeneous population of neural stem cells which were previously derived from embryonic stem cells as well as from the fetal and adult brain. Our previous reports have described a 21 day long neuronal differentiation protocol able to reproducibly convert adult SVZ-derived NS (aNS) cells into a population composed of 65% mature neurons and 35% glial cells. Here we have developed a different procedure specifically applicable to ES-derived NS cells in order to fully explore their neurogenic capacity. Differently from the aNS differentiation procedure, optimized neuronal output from ES-derived NS cells requires replating of the cells on appropriate substrates followed by sequential exposure to modified media. In these conditions, ES-derived NS cells differentiate into neurons with a barely appreciable quota of astrocytes and occasional oligodendrocytes. In particular, 21 days after the beginning of the treatment, 85% of the cells has differentiated into molecularly and electrophysiologically mature neurons belonging to the GABAergic lineage. The procedure, which is applicable with no considerable differences to different ES-derived NS cell lines and to NS cells at different passages, opens to the possibility of molecular and biochemical studies on close-to-uniform stem cell derived neurons.
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| Titolo: | An optimized experimental strategy for efficient conversion of embryonic stem (ES)-derived mouse neural stem (NS) cells into a nearly homogeneous mature neuronal population. | |
| Autori: | Spiliotopoulos, D; Goffredo, D; Conti, Luciano; Di Febo, F; Biella, G; Toselli, M; Cattaneo, E. | |
| Autori Unitn: | ||
| Titolo del periodico: | NEUROBIOLOGY OF DISEASE | |
| Anno di pubblicazione: | 2009 | |
| Numero e parte del fascicolo: | 2 | |
| Codice identificativo Scopus: | 2-s2.0-64349101680 | |
| Codice identificativo Pubmed: | 19236914 | |
| Codice identificativo WOS: | WOS:000265869400013 | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.nbd.2009.02.007 | |
| Handle: | http://hdl.handle.net/11572/99130 | |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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