Wnt5a is a transcriptional target of Dlx homeogenes and promotes differentiation of interneuron progenitors in vitro and in vivo.

During brain development, neurogenesis, migration, and differentiation of neural progenitor cells are regulated by an interplay between intrinsic genetic programs and extrinsic cues. The Dlx homeogene transcription factors have been proposed to directly control the genesis and maturation of GABAergic interneurons of the olfactory bulb (OB), subpallium, and cortex. Here we provide evidence that Dlx genes promote differentiation of olfactory interneurons via the signaling molecule Wnt5a. Dlx2 and Dlx5 interact with homeodomain binding sequences within the Wnt5a locus and activate its transcription. Exogenously provided Wnt5a promotes GABAergic differentiation in dissociated OB neurons and in organ-type brain cultures. Finally, we show that the Dlx-mutant environment is unfavorable for GABA differentiation, in vivo and in vitro. We conclude that Dlx genes favor interneuron differentiation also in a non-cell-autonomous fashion, via expression of Wnt5a.



Titolo: Wnt5a is a transcriptional target of Dlx homeogenes and promotes differentiation of interneuron progenitors in vitro and in vivo.
Autori: Paina, S; Garzotto, D; Demarchis, S; Marino, M; Moiana, A; Conti, Luciano; Cattaneo, E; Perera, M; Corte, G; Calautti, E; Merlo, Gr
Autori Unitn: 
Conti, Luciano
mostra contributor esterni 
Titolo del periodico: THE JOURNAL OF NEUROSCIENCE
Anno di pubblicazione: 2011
Numero e parte del fascicolo: 7
Codice identificativo Scopus: 2-s2.0-79951828066
Codice identificativo Pubmed: 21325536
Codice identificativo ISI: WOS:000287392400037
Digital Object Identifier (DOI): http://dx.doi.org/10.1523/JNEUROSCI.3110-10.2011
Handle: http://hdl.handle.net/11572/99124
Appare nelle tipologie:03.1 Articolo su rivista (Journal article)

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