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EnglishIn the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
| Titolo: | Paramagnetism-Based Refinement Strategy for the Solution Structure of Human α-Parvalbumin |
| Autori: | I., Baig; I., Bertini; Del Bianco, Cristina; Y. K., Gupta; Y., Min Lee; C., Luchinat; Quattrone, Alessandro |
| Autori Unitn: | |
| Titolo del periodico: | BIOCHEMISTRY |
| Anno di pubblicazione: | 2004 |
| Codice identificativo Pubmed: | 15122922 |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1021/bi035879k |
| Handle: | http://hdl.handle.net/11572/96521 |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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