This work presents an improved platform for single-site electroporation and controlled transfectants’ delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface. The system has been previously tested by electroporating them with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmids for the enhanced expression of Green Fluorescence Protein (pEGFP) delivered through microchannels.

An enhanced platform for cell electroporation: controlled delivery and electrodes functionalization

Vidalino, Laura;Macchi, Paolo
2010

Abstract

This work presents an improved platform for single-site electroporation and controlled transfectants’ delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface. The system has been previously tested by electroporating them with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmids for the enhanced expression of Green Fluorescence Protein (pEGFP) delivered through microchannels.
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L., Odorizzia; C., Ressa; C., Collini; E., Morganti; L., Lorenzelli; N., Coppedé; A. B., Alabi; S., Iannotta; Vidalino, Laura; Macchi, Paolo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/93356
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