This work presents an improved platform for single-site electroporation and controlled transfectants delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO 2) functionalized electrodes for the improvement of cell adhesion. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface using traditional protocols. The system has been previously tested by electroporating HeLa cells with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmid for the enhanced expression of Green Fluorescence Protein (pEGFP-N1) delivered through microchannels
An integrated platform for in vitro single-site cell electroporation: Controlled delivery and electrodes functionalization / Odorizzi, L., Ress, C., Collini, C., Morrganti, E., Lorenzelli, L., Coppedè, N., Alabi, A.B., Iannotta, S., Cazzanelli, E., Vidalino, L., Macchi, P.. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 170:170(2012), pp. 182-188. [10.1016/j.snb.2011.06.027]
An integrated platform for in vitro single-site cell electroporation: Controlled delivery and electrodes functionalization
Ress, C.;Lorenzelli, L.;Coppedè, N.;Cazzanelli, E.;Vidalino, L.;Macchi, P.
2012-01-01
Abstract
This work presents an improved platform for single-site electroporation and controlled transfectants delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO 2) functionalized electrodes for the improvement of cell adhesion. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface using traditional protocols. The system has been previously tested by electroporating HeLa cells with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmid for the enhanced expression of Green Fluorescence Protein (pEGFP-N1) delivered through microchannels| File | Dimensione | Formato | |
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