Here we present a novel matrix-mimetic nanoassembly based on polysaccharides. Chitosan electrospun fiber networks are decorated with heparin-containing polyelectrolyte complex nanoparticles (PCNs) that present basic fibroblast growth factor (FGF-2), both stably adsorbed to the surfaces and released into solution. These FGF-2/PCN complexes can be released from the fibers with zero-order kinetics over a period of 30 days. Further modification of fibers with a single bilayer of polyelectrolyte multilayer (PEM) composed of N,N,N-trimethyl chitosan and heparin completely prevent release, and the FGF-2/PCN complexes are retained on the fibers for the duration of the release experiment (30 days). We also compare the mitogenic activity of these FGF-2/PCN complexes delivered in two different states: adsorbed to a surface and dissolved in solution. FGF-2/PCN complexes exhibit mitogenic activity with respect to ovine bone marrow-derived mesenchymal stem cells, even after being preconditioned by incubating for 27 days at 37 C in solution. However, when the FGF-2/PCN complexes are adsorbed to chitosan and coated with PEMs, the mitogenic activity of the FGF-2 steadily decreases with increasing preconditioning time. This work demonstrates a new system for stabilizing and controlling the delivery of heparinbinding growth factors, using polysaccharide-based matrix-mimetic nanomaterials. This work also contributes to our understanding of the preferred mode of growth factor delivery from porous scaffolds. © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Preservation of FGF-2 Bioactivity Using Heparin-Based Nanoparticles, and their Delivery from Electrospun Chitosan Fibers / Zomer Volpato, Fabio; J., Almodovar; K., Erickson; K. C., Popat; Migliaresi, Claudio; M., Kipper. - In: ACTA BIOMATERIALIA. - ISSN 1742-7061. - STAMPA. - 8:4(2012), pp. 1551-1559.

Preservation of FGF-2 Bioactivity Using Heparin-Based Nanoparticles, and their Delivery from Electrospun Chitosan Fibers

Zomer Volpato, Fabio;Migliaresi, Claudio;
2012-01-01

Abstract

Here we present a novel matrix-mimetic nanoassembly based on polysaccharides. Chitosan electrospun fiber networks are decorated with heparin-containing polyelectrolyte complex nanoparticles (PCNs) that present basic fibroblast growth factor (FGF-2), both stably adsorbed to the surfaces and released into solution. These FGF-2/PCN complexes can be released from the fibers with zero-order kinetics over a period of 30 days. Further modification of fibers with a single bilayer of polyelectrolyte multilayer (PEM) composed of N,N,N-trimethyl chitosan and heparin completely prevent release, and the FGF-2/PCN complexes are retained on the fibers for the duration of the release experiment (30 days). We also compare the mitogenic activity of these FGF-2/PCN complexes delivered in two different states: adsorbed to a surface and dissolved in solution. FGF-2/PCN complexes exhibit mitogenic activity with respect to ovine bone marrow-derived mesenchymal stem cells, even after being preconditioned by incubating for 27 days at 37 C in solution. However, when the FGF-2/PCN complexes are adsorbed to chitosan and coated with PEMs, the mitogenic activity of the FGF-2 steadily decreases with increasing preconditioning time. This work demonstrates a new system for stabilizing and controlling the delivery of heparinbinding growth factors, using polysaccharide-based matrix-mimetic nanomaterials. This work also contributes to our understanding of the preferred mode of growth factor delivery from porous scaffolds. © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
2012
4
Zomer Volpato, Fabio; J., Almodovar; K., Erickson; K. C., Popat; Migliaresi, Claudio; M., Kipper
Preservation of FGF-2 Bioactivity Using Heparin-Based Nanoparticles, and their Delivery from Electrospun Chitosan Fibers / Zomer Volpato, Fabio; J., Almodovar; K., Erickson; K. C., Popat; Migliaresi, Claudio; M., Kipper. - In: ACTA BIOMATERIALIA. - ISSN 1742-7061. - STAMPA. - 8:4(2012), pp. 1551-1559.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/93161
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