CINECA IRIS Institutional Research Information System
IRIS è l'Anagrafe della ricerca dell'Università degli Studi di Trento, e ha lo scopo di raccogliere, documentare, conservare e diffondere, anche ad accesso aperto, la produzione scientifica degli autori afferenti all'Università degli Studi di Trento.
EnglishThe crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 Å shortening of the Fe−O bond (relative to Fe−S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe−S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.
Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster / Mansy, Sheref Samir; Y., Xiong; C., Hemann; R., Hille; M., Sundaralingam; J. A., Cowan. - In: BIOCHEMISTRY. - ISSN 0006-2960. - STAMPA. - 41:4(2002), pp. 1195-1201.
| Titolo: | Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster. |
| Autori: | Mansy, Sheref Samir; Y., Xiong; C., Hemann; R., Hille; M., Sundaralingam; J. A., Cowan |
| Autori Unitn: | |
| Titolo del periodico: | BIOCHEMISTRY |
| Anno di pubblicazione: | 2002 |
| Numero e parte del fascicolo: | 4 |
| Codice identificativo Scopus: | 2-s2.0-0037192119 |
| Codice identificativo Pubmed: | 11802718 |
| Codice identificativo ISI: | WOS:000173612100012 |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1021/bi011811y |
| Handle: | http://hdl.handle.net/11572/89671 |
| Citazione: | Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster / Mansy, Sheref Samir; Y., Xiong; C., Hemann; R., Hille; M., Sundaralingam; J. A., Cowan. - In: BIOCHEMISTRY. - ISSN 0006-2960. - STAMPA. - 41:4(2002), pp. 1195-1201. |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 2002MansyBiochemistry.pdf | Versione editoriale (Publisher’s layout) | Tutti i diritti riservati (All rights reserved) | Administrator |
Copyright © 2021