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EnglishIscU functions as a scaffold for Fe-S cluster assembly and transfer, and is known to be a substrate protein for molecular chaperones. Kinetic studies of Fe-S cluster transfer from holo IscU to apo Fd in the presence of chaperone DnaK demonstrate an inhibitory effect on the rate of Fe-S cluster transfer from IscU. Binding of DnaK reduces the rate of formation of the IscU−Fd complex (greater than 8-fold), but has little influence on the intrinsic rate of iron−sulfur cluster transfer to apo Fd. Apparently the molecular chaperone DnaK does not facilitate the process of Fe-S cluster transfer from IscU. Rather, DnaK has a modest influence on the stability of the IscU-bound Fe-S cluster that may reflect a more important role in promoting cluster assembly. In accord with prior observations the cochaperone DnaJ stimulates the ATPase activity of DnaK, but has a minimal influence on IscU cluster transfer activity, either alone or in concert with DnaK.
| Titolo: | Iron-sulfur cluster biosynthesis. Molecular chaperone DnaK promotes IscU-bound [2Fe-2S] cluster stability and inhibits cluster transfer activity. |
| Autori: | S. P., Wu; Mansy, Sheref Samir; J. A., Cowan |
| Autori Unitn: | |
| Titolo del periodico: | BIOCHEMISTRY |
| Anno di pubblicazione: | 2005 |
| Numero e parte del fascicolo: | 11 |
| Codice identificativo Scopus: | 2-s2.0-15544365805 |
| Codice identificativo Pubmed: | 15766257 |
| Codice identificativo ISI: | WOS:000227736900017 |
| Digital Object Identifier (DOI): | 10.1021/bi0483007 |
| Handle: | http://hdl.handle.net/11572/89668 |
| Citazione: | Iron-sulfur cluster biosynthesis. Molecular chaperone DnaK promotes IscU-bound [2Fe-2S] cluster stability and inhibits cluster transfer activity / S. P., Wu; Mansy, Sheref Samir; J. A., Cowan. - In: BIOCHEMISTRY. - ISSN 0006-2960. - STAMPA. - 44:11(2005), pp. 4284-4293. |
| Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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