The main goal in the last few years in cardiac research has been to isolate cardiac potential stem cells from adult myocardium and to demonstrate their differentiation potential. We have previously demonstrated that c-Kit positive cardiac stem cells are able to organize themselves into a tissue-like cell mass. In this 3D mass, they can produce a high concentration of natural extracellular matrix, can create vessels, a capsule and, with the help of an Open-pore Polylactic Acid scaffold, many cells can organize an elementary myocardium. Drawing from this background, we decided to design and use poly-lactic scaffolds and the model of the athymic Nude-Foxn1nu mouse to evaluate the extent of the myogenic vs endothelial differentiation in vivo, and to evaluate the presence or the absence of a foreign body reaction.
Titolo: | Adult stem cells, scaffold for in vivo and in vitro myocardial tissue engineering |
Autori Unitn: | |
Anno di pubblicazione: | 2010 |
Titolo del periodico: | |
Citazione: | Adult stem cells, scaffold for in vivo and in vitro myocardial tissue engineering / V., Di Felice; A., De Luca; C., Serrafico; P., Di Marco; L., Verin; Motta, Antonella; A., Guercio; G., Zummo. - In: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY. - ISSN 1122-6714. - STAMPA. - 115:1-2(2010), pp. 65-69. |
Abstract: | The main goal in the last few years in cardiac research has been to isolate cardiac potential stem cells from adult myocardium and to demonstrate their differentiation potential. We have previously demonstrated that c-Kit positive cardiac stem cells are able to organize themselves into a tissue-like cell mass. In this 3D mass, they can produce a high concentration of natural extracellular matrix, can create vessels, a capsule and, with the help of an Open-pore Polylactic Acid scaffold, many cells can organize an elementary myocardium. Drawing from this background, we decided to design and use poly-lactic scaffolds and the model of the athymic Nude-Foxn1nu mouse to evaluate the extent of the myogenic vs endothelial differentiation in vivo, and to evaluate the presence or the absence of a foreign body reaction. |
Handle: | http://hdl.handle.net/11572/89569 |
Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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