We have developed FusionSeq to identify fusion transcripts from paired-end RNA-sequencing. FusionSeq includes filters to remove spurious candidate fusions with artifacts, such as misalignment or random pairing of transcript fragments, and it ranks candidates according to several statistics. It also has a module to identify exact sequences at breakpoint junctions. FusionSeq detected known and novel fusions in a specially sequenced calibration data set, including eight cancers with and without known rearrangements.

FusionSeq: a modular framework for finding gene fusions by analyzing paired-end RNA-sequencing data.

Demichelis, Francesca;
2010-01-01

Abstract

We have developed FusionSeq to identify fusion transcripts from paired-end RNA-sequencing. FusionSeq includes filters to remove spurious candidate fusions with artifacts, such as misalignment or random pairing of transcript fragments, and it ranks candidates according to several statistics. It also has a module to identify exact sequences at breakpoint junctions. FusionSeq detected known and novel fusions in a specially sequenced calibration data set, including eight cancers with and without known rearrangements.
2010
A., Sboner; L., Habegger; D., Pflueger; S., Terry; D. Z., Chen; J. S., Rozowsky; A. K., Tewari; N., Kitabayashi; B. J., Moss; M. S., Chee; Demichelis, Francesca; M. A., Rubin; M. B., Gerstein
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/88959
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