The construction of genetically encoded cellular mimics in compartments containing organized synthetic cytosols is desirable for the development of artificial cells. Phase separated aqueous domains were placed within water-in-oil emulsion droplets in a manner compatible with transcription and translation machinery. Aqueous two-phase and three-phase systems (ATPS and A3PS) were assembled with dextran, poly(ethylene glycol), and Ficoll. Aqueous two-phase systems were capable of supporting the cell-free expression of protein within water droplets, whereas the aqueous three-phase-based system did not give rise to detectable protein synthesis. The expressed protein preferentially partitioned to the dextran-enriched phase. The system could serve as a foundation for building cellular mimics with liquid organelles.

Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription–Translation / Torre, Paola; Keating, Christine D.; Mansy, Sheref Samir. - In: LANGMUIR. - ISSN 0743-7463. - STAMPA. - 30:(2014), pp. 5695-5699. [10.1021/la404146g]

Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription–Translation

Torre, Paola;Mansy, Sheref Samir
2014-01-01

Abstract

The construction of genetically encoded cellular mimics in compartments containing organized synthetic cytosols is desirable for the development of artificial cells. Phase separated aqueous domains were placed within water-in-oil emulsion droplets in a manner compatible with transcription and translation machinery. Aqueous two-phase and three-phase systems (ATPS and A3PS) were assembled with dextran, poly(ethylene glycol), and Ficoll. Aqueous two-phase systems were capable of supporting the cell-free expression of protein within water droplets, whereas the aqueous three-phase-based system did not give rise to detectable protein synthesis. The expressed protein preferentially partitioned to the dextran-enriched phase. The system could serve as a foundation for building cellular mimics with liquid organelles.
2014
Torre, Paola; Keating, Christine D.; Mansy, Sheref Samir
Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription–Translation / Torre, Paola; Keating, Christine D.; Mansy, Sheref Samir. - In: LANGMUIR. - ISSN 0743-7463. - STAMPA. - 30:(2014), pp. 5695-5699. [10.1021/la404146g]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/68721
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