Ethylene is a plant hormone widely used to ripen fruit. However, the synthesis, handling, and storage of ethylene are environmentally harmful and dangerous. We engineered E. coli to produce ethylene through the activity of the ethylene-forming enzyme (EFE) from Pseudomonas syringae. EFE converts a citric acid cycle intermediate, 2-oxoglutarate, to ethylene in a single step. The production of ethylene was placed under the control of arabinose and blue light responsive regulatory systems. The resulting bacteria were capable of accelerating the ripening of tomatoes, kiwifruit, and apples.

Ethylene-producing bacteria that ripen fruit / Digiacomo, F; Girelli, G; Aor, B; Marchioretti, C; Pedrotti, M; Perli, T; Tonon, E; Valentini, V; Avi, Damiano; Ferrentino, G; Dorigato, Andrea; Jousson, Olivier; Mansy, Sheref Samir; Del Bianco, Cristina. - In: ACS SYNTHETIC BIOLOGY. - ISSN 2161-5063. - 3:12(2014), pp. 935-938. [10.1021/sb5000077]

Ethylene-producing bacteria that ripen fruit

Avi, Damiano;Dorigato, Andrea;Jousson, Olivier;Mansy, Sheref Samir;Del Bianco, Cristina
2014-01-01

Abstract

Ethylene is a plant hormone widely used to ripen fruit. However, the synthesis, handling, and storage of ethylene are environmentally harmful and dangerous. We engineered E. coli to produce ethylene through the activity of the ethylene-forming enzyme (EFE) from Pseudomonas syringae. EFE converts a citric acid cycle intermediate, 2-oxoglutarate, to ethylene in a single step. The production of ethylene was placed under the control of arabinose and blue light responsive regulatory systems. The resulting bacteria were capable of accelerating the ripening of tomatoes, kiwifruit, and apples.
2014
12
Digiacomo, F; Girelli, G; Aor, B; Marchioretti, C; Pedrotti, M; Perli, T; Tonon, E; Valentini, V; Avi, Damiano; Ferrentino, G; Dorigato, Andrea; Jousson, Olivier; Mansy, Sheref Samir; Del Bianco, Cristina
Ethylene-producing bacteria that ripen fruit / Digiacomo, F; Girelli, G; Aor, B; Marchioretti, C; Pedrotti, M; Perli, T; Tonon, E; Valentini, V; Avi, Damiano; Ferrentino, G; Dorigato, Andrea; Jousson, Olivier; Mansy, Sheref Samir; Del Bianco, Cristina. - In: ACS SYNTHETIC BIOLOGY. - ISSN 2161-5063. - 3:12(2014), pp. 935-938. [10.1021/sb5000077]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/67637
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