This study was designed to investigate whether culture conditions (media, seawater concn. and pH) could lead Streptomyces sundarbansensis strain (isolated from marine brown algae Fucus sp. collected from Algerian coastline) to produce bioactive secondary metabolites. The most favorable condition for the prodn. of anti-MRSA compd. 1 [2- hydroxy-5-((6-hydroxy-4-oxo-4H-pyran-2-yl)methyl)-2-propylchroman-4-one] was detd. Methods and Results : The profile of metabolites present in the crude exts. was carried out by HPLC anal. equipped with a diode array detector evaporative light scattering detection (DAD-ELSD) or online coupled to electrospray ionization-mass spectrometry (ESIMS). Compd. 1 was the most abundant secondary metabolite by culturing the strains on starch casein agar (SCA) medium in freshwater or 50% seawater at pH 7 or 9 using agar-state fermn. method. Conclusions : The study has shown the efficiency of HPLC/ESI-MS technique in the anal. of polyketides produced by the strain under investigation. It was possible to establish the best culture conditions for obtaining the most bioactive compd. 1, previously isolated by the same strain. Significance and Impact of the Study : Marine algae-actinobacteria assocns. are a particularly promising renewable system for the prodn. of new antibacterial metabolites. Based on the promising bioactivity of the chem. characterized compd. 1, the anal. methodol. here applied has resulted as an effective approach for establishing its optimized prodn.
Metabolite profile of marine-derived endophyticStreptomyces sundarbansensis WR1L1S8by liquid chromatography-mass spectrometry and evaluation of culture conditions on antibacterial activity and mycelial growth
Defant, Andrea;Mancini, Ines
2014-01-01
Abstract
This study was designed to investigate whether culture conditions (media, seawater concn. and pH) could lead Streptomyces sundarbansensis strain (isolated from marine brown algae Fucus sp. collected from Algerian coastline) to produce bioactive secondary metabolites. The most favorable condition for the prodn. of anti-MRSA compd. 1 [2- hydroxy-5-((6-hydroxy-4-oxo-4H-pyran-2-yl)methyl)-2-propylchroman-4-one] was detd. Methods and Results : The profile of metabolites present in the crude exts. was carried out by HPLC anal. equipped with a diode array detector evaporative light scattering detection (DAD-ELSD) or online coupled to electrospray ionization-mass spectrometry (ESIMS). Compd. 1 was the most abundant secondary metabolite by culturing the strains on starch casein agar (SCA) medium in freshwater or 50% seawater at pH 7 or 9 using agar-state fermn. method. Conclusions : The study has shown the efficiency of HPLC/ESI-MS technique in the anal. of polyketides produced by the strain under investigation. It was possible to establish the best culture conditions for obtaining the most bioactive compd. 1, previously isolated by the same strain. Significance and Impact of the Study : Marine algae-actinobacteria assocns. are a particularly promising renewable system for the prodn. of new antibacterial metabolites. Based on the promising bioactivity of the chem. characterized compd. 1, the anal. methodol. here applied has resulted as an effective approach for establishing its optimized prodn.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione
