Protocol for assessing visual stimulus discrimination in Danionella cerebrum and Danio rerio using habituation/dishabituation

Here, we present a protocol to assess visual discrimination in fish, specifically Danionella cerebrum and Danio rerio . We describe steps for the familiarization of subjects to reduce contextual novelty and habituation through repeated presentation of a constant feature stimulus. We then detail procedures for a dishabituation session that introduces a stimulus change. In this protocol, the recovery of behavioral response provides a measure of change detection and feature discrimination. For complete details on the use and execution of this protocol, please refer to Zanon et al. 1