Over the past two decades, numerous techniques have been developed for analysing microRNAs in body fluids and tissues. However, these techniques still face technical challenges, particularly when compared to well-established techniques for proteins and metabolites. Recently, the ODG platform was introduced, which is an innovative technology that allows for the direct detection and quantification of microRNAs in liquid biopsies without requiring extraction or amplification. This study presents the implementation of the ODG platform within a semi-automated protocol to create the “SA-ODG” platform, enhancing the efficiency and precision of microRNA testing while reducing hands-on time required by laboratory staff. For the first time, the SA-ODG platform has been used to directly quantify microRNAs in solid tissues. The results demonstrate precise analysis of miR-122-5p in mouse liver tissues using SA-ODG. These developments represent a crucial step forward in advancing the field of extraction and amplification-free microRNA detection and quantification.

SA-ODG platform: a semi-automated and PCR-free method to analyse microRNAs in solid tissues / Detassis, S.; Precazzini, F.; Brentari, I.; Ruffilli, R.; Ress, C.; Maglione, A.; Pernagallo, S.; Denti, M. A.. - In: ANALYST. - ISSN 0003-2654. - ELETTRONICO. - 149:15(2024), pp. 3891-3899. [10.1039/d4an00783b]

SA-ODG platform: a semi-automated and PCR-free method to analyse microRNAs in solid tissues

Detassis, S.
Primo
;
Precazzini, F.
Secondo
;
Brentari, I.;Ress, C.;Maglione, A.;Pernagallo, S.;Denti, M. A.
Ultimo
2024-01-01

Abstract

Over the past two decades, numerous techniques have been developed for analysing microRNAs in body fluids and tissues. However, these techniques still face technical challenges, particularly when compared to well-established techniques for proteins and metabolites. Recently, the ODG platform was introduced, which is an innovative technology that allows for the direct detection and quantification of microRNAs in liquid biopsies without requiring extraction or amplification. This study presents the implementation of the ODG platform within a semi-automated protocol to create the “SA-ODG” platform, enhancing the efficiency and precision of microRNA testing while reducing hands-on time required by laboratory staff. For the first time, the SA-ODG platform has been used to directly quantify microRNAs in solid tissues. The results demonstrate precise analysis of miR-122-5p in mouse liver tissues using SA-ODG. These developments represent a crucial step forward in advancing the field of extraction and amplification-free microRNA detection and quantification.
2024
15
Settore BIO/13 - Biologia Applicata
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore BIOS-10/A - Biologia cellulare e applicata
Settore BIOS-09/A - Biochimica clinica e biologia molecolare clinica
Detassis, S.; Precazzini, F.; Brentari, I.; Ruffilli, R.; Ress, C.; Maglione, A.; Pernagallo, S.; Denti, M. A.
SA-ODG platform: a semi-automated and PCR-free method to analyse microRNAs in solid tissues / Detassis, S.; Precazzini, F.; Brentari, I.; Ruffilli, R.; Ress, C.; Maglione, A.; Pernagallo, S.; Denti, M. A.. - In: ANALYST. - ISSN 0003-2654. - ELETTRONICO. - 149:15(2024), pp. 3891-3899. [10.1039/d4an00783b]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/446990
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