The emergence of new SARS-CoV-2 variants poses challenges to global surveillance eforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 and its sub-lineages have gained attention due to their potential immune evasion properties. This study describes the development of a digital PCR assay for the rapid detection of BA.2.86 and its descendant lineages, in wastewater samples. By using this assay, we analyzed wastewater samples collected in Italy from September 2023 to January 2024. Our analysis revealed the presence of BA.2.86 lineages already in October 2023 with a minimal detection rate of 2% which then rapidly increased, becoming dominant by January 2024, accounting for a prevalence of 62%. The fndings emphasize the signifcance of wastewater-based surveillance in tracking emerging variants and underscore the efcacy of targeted digital PCR assays for environmental monitoring.
Tracking the Spread of the BA.2.86 Lineage in Italy through Wastewater Analysis / Veneri, C.; Brandtner, D.; Mancini, P.; Bonanno Ferraro, G.; Iaconelli, M.; Suffredini, E.; Petrillo, M.; Leoni, G.; Paracchini, V.; Gawlik, B. M.; Marchini, A.; Torlontano, Paolo; Aprea, Giuseppe; Scattolini, Silvia; Vicdalia Aniela Acciari, ; Michele La Bianca, ; Rosa Anna Cifarelli, ; Palma, Achille; Lauria, Giuseppe; Giovanna La Vecchia, ; Giordano, Vincenzo; Cossentino, Luigi; Pennino, Francesca; Lombardi, Annalisa; Gentili, Lisa; Angelini, Paola; Nasci, Daniele; Alborali, Giovanni; Formenti, Nicoletta; Guarneri, Flavia; Fontani, Nadia; Guercio, Marco; Mariuz, Marika; Trani, Gabriella; Pariani, Anna; Laura De Lellis, ; Ancona, Carla; Barca, Alessandra; Serio, Flavia; Doriana Antonella Giorgi, ; Ferrante, Irene; Capparuccini, Valeria; Maria Teresa Scicluna, ; Cersini, Antonella; Pietrella, Gabriele; Nicosia, Elena; Fontani, Nadia; Guercio, Marco; Grasselli, Elena; Izzotti, Alberto; Tomesani, Irene; Bellisomi, Marta; Rosatto, Stefano; Ammoni, Emanuela; Cereda, Danilo; Bertasi, Barbara; Marina Nadia Losio, ; Oliva, Desdemona; Castiglioni, Sara; Schiarea, Silvia; Binda, Sandro; Primache, Valeria; Pellegrinelli, Laura; Cocuzza, Clementina; Musumeci, Rosario; Bolognini, Luigi; Filippetti, Fabio; Paniccia’, Marta; Briscolini, Sara; Magi, Silvia; Grucci, Annalisa; Colitti, Michele; Ciccaglione, Angela; Montanaro, Carmen; Griglio, Bartolomeo; Costa, Angela; Decastelli, Lucia; Romano, Angelo; Bianchi, Manila; Carraro, Elisabetta; Pignata, Cristina; Macrì, Manuela; Bonetta, Silvia; Albano, Nehludoff; Giuseppe Di Vittorio, ; Mongelli, Onofrio; Apollonio, Francesca; Triggiano, Francesco; Osvalda De Giglio, ; Maria Teresa Montagna, ; Palermo, Mario; Carmelo Massimo Maida, ; Mazzucco, Walter; Tramuto, Fabio; Simona De Grazia, ; Giovanni Maurizio Giammanco, ; Filizzolo, Chiara; Purpari, Giuseppa; Gucciardi, Francesca; Ferrante, Margherita; Agodi, Antonella; Barchitta, Martina; Cala’, Piergiuseppe; Carducci, Annalaura; Verani, Marco; Federigi, Ileana; Macrì, Salvatore; Federici, Ermanno; Petricciuolo, Maya; Carnevali, Agnese; Russo, Francesca; Pitter, Gisella; Groppi, Vanessa; Rigoli, Franco; Zampini, Marco; Baldovin, Tatjana; Amoruso, Irene; Cadonna, Maria; Postinghel, Mattia; Foladori, Paola; Zago, Lorella; Stenico, Alberta; Morelli, Marco; Matteo, Dossena; La Rosa, G.. - In: FOOD AND ENVIRONMENTAL VIROLOGY. - ISSN 1867-0334. - 2024:(2024), pp. 1-9. [10.1007/s12560-024-09607-1]
Tracking the Spread of the BA.2.86 Lineage in Italy through Wastewater Analysis
C. Veneri;Giuseppe Aprea;Paola Foladori;Morelli Marco;
2024-01-01
Abstract
The emergence of new SARS-CoV-2 variants poses challenges to global surveillance eforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 and its sub-lineages have gained attention due to their potential immune evasion properties. This study describes the development of a digital PCR assay for the rapid detection of BA.2.86 and its descendant lineages, in wastewater samples. By using this assay, we analyzed wastewater samples collected in Italy from September 2023 to January 2024. Our analysis revealed the presence of BA.2.86 lineages already in October 2023 with a minimal detection rate of 2% which then rapidly increased, becoming dominant by January 2024, accounting for a prevalence of 62%. The fndings emphasize the signifcance of wastewater-based surveillance in tracking emerging variants and underscore the efcacy of targeted digital PCR assays for environmental monitoring.| File | Dimensione | Formato | |
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