Objective. To evaluate intra-observer diagnostic reproducibility using traditional slides (TS) versus whole slide images (WSI). Methods. TS and WSI of 1427 prostatic biopsies (107 consecutive patients) were evaluated by a single pathologist. Agreement between readings was evaluated with Gwet’s Agreement coefficient (AC) and Landis and Koch benchmark scale. Results. The positive/negative agreement between the readings was almost perfect (AC1= 0.962; 95% CI[0.949,0.974]), with method independent distribution of discrepancies. Among positive biopsies, 212 had identical Gleason score (GS) on TS and WSI and discordant GS in 69 cases (AC2 = 0.932; 95% CI[0.907, 0.956]). Concordant negative and positive patient classification was observed in 39 and 64 cases, respectively; two cases were assigned to the positive group on TS and 2 on WSI configuring an almost perfect agreement (AC1=0.929; 95% C1[0.860, 0.998]). ISUP Grade group (ISUP GG) agreement was evaluated in the 60 concordantly positive cases: in 45 cases it was identical on TS and WSI; in 10 biopsies the discrepancy implied a modification of the assigned ISUP GG of ≤ 1 class and in 5 the discrepancy implied a modification of 2 classes. Gwet’s agreement coefficient was (95% CI [0.834, 0.962]), i. e.: almost perfect agreement. Conclusions. Our data show almost perfect agreement between digital and traditional diagnostic activity in a routine setting, confirming that digital pathology can be safely introduced into routine workflows.

Diagnostic concordance between traditional and digital workflows. A study on 1427 prostate biopsies / Torresani, Evelin; Gentilini, Maria; Adalgisa, ; Grassi, Stefano; Cima, Luca; Pedrolli, Irene; Cai, Tommaso; Puglisi, Marco; Vattovani, Valentino; Guadin, Bianca; Brunelli, Matteo; Doglioni, Claudio; Barbareschi, Mattia. - In: PATHOLOGICA. - ISSN 1591-951X. - 115:4(2023), pp. 221-226. [10.32074/1591-951X-896]

Diagnostic concordance between traditional and digital workflows. A study on 1427 prostate biopsies

Brunelli, Matteo;Doglioni, Claudio;Barbareschi, Mattia
2023-01-01

Abstract

Objective. To evaluate intra-observer diagnostic reproducibility using traditional slides (TS) versus whole slide images (WSI). Methods. TS and WSI of 1427 prostatic biopsies (107 consecutive patients) were evaluated by a single pathologist. Agreement between readings was evaluated with Gwet’s Agreement coefficient (AC) and Landis and Koch benchmark scale. Results. The positive/negative agreement between the readings was almost perfect (AC1= 0.962; 95% CI[0.949,0.974]), with method independent distribution of discrepancies. Among positive biopsies, 212 had identical Gleason score (GS) on TS and WSI and discordant GS in 69 cases (AC2 = 0.932; 95% CI[0.907, 0.956]). Concordant negative and positive patient classification was observed in 39 and 64 cases, respectively; two cases were assigned to the positive group on TS and 2 on WSI configuring an almost perfect agreement (AC1=0.929; 95% C1[0.860, 0.998]). ISUP Grade group (ISUP GG) agreement was evaluated in the 60 concordantly positive cases: in 45 cases it was identical on TS and WSI; in 10 biopsies the discrepancy implied a modification of the assigned ISUP GG of ≤ 1 class and in 5 the discrepancy implied a modification of 2 classes. Gwet’s agreement coefficient was (95% CI [0.834, 0.962]), i. e.: almost perfect agreement. Conclusions. Our data show almost perfect agreement between digital and traditional diagnostic activity in a routine setting, confirming that digital pathology can be safely introduced into routine workflows.
2023
4
Torresani, Evelin; Gentilini, Maria; Adalgisa, ; Grassi, Stefano; Cima, Luca; Pedrolli, Irene; Cai, Tommaso; Puglisi, Marco; Vattovani, Valentino; Gua...espandi
Diagnostic concordance between traditional and digital workflows. A study on 1427 prostate biopsies / Torresani, Evelin; Gentilini, Maria; Adalgisa, ; Grassi, Stefano; Cima, Luca; Pedrolli, Irene; Cai, Tommaso; Puglisi, Marco; Vattovani, Valentino; Guadin, Bianca; Brunelli, Matteo; Doglioni, Claudio; Barbareschi, Mattia. - In: PATHOLOGICA. - ISSN 1591-951X. - 115:4(2023), pp. 221-226. [10.32074/1591-951X-896]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/411454
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