West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel wholegenome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.

Novel Amplicon-Based Sequencing Approach to West Nile Virus / Diagne, Moussa Moïse; Ndione, Marie Henriette Dior; Mencattelli, Giulia; Diallo, Amadou; Ndiaye, El hadji; Di Domenico, Marco; Diallo, Diawo; Kane, Mouhamed; Curini, Valentina; Top, Ndeye Marieme; Marcacci, Maurilia; Mbanne, Maïmouna; Ancora, Massimo; Secondini, Barbara; Di Lollo, Valeria; Teodori, Liana; Leone, Alessandra; Puglia, Ilaria; Gaye, Alioune; Sall, Amadou Alpha; Loucoubar, Cheikh; Rosà, Roberto; Diallo, Mawlouth; Monaco, Federica; Faye, Ousmane; Cammà, Cesare; Rizzoli, Annapaola; Savini, Giovanni; Faye, Oumar. - In: VIRUSES. - ISSN 1999-4915. - 15:6(2023), pp. 126101-126115. [10.3390/v15061261]

Novel Amplicon-Based Sequencing Approach to West Nile Virus

Mencattelli, Giulia;Rosà, Roberto;
2023-01-01

Abstract

West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel wholegenome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.
2023
6
Diagne, Moussa Moïse; Ndione, Marie Henriette Dior; Mencattelli, Giulia; Diallo, Amadou; Ndiaye, El hadji; Di Domenico, Marco; Diallo, Diawo; Kane, Mo...espandi
Novel Amplicon-Based Sequencing Approach to West Nile Virus / Diagne, Moussa Moïse; Ndione, Marie Henriette Dior; Mencattelli, Giulia; Diallo, Amadou; Ndiaye, El hadji; Di Domenico, Marco; Diallo, Diawo; Kane, Mouhamed; Curini, Valentina; Top, Ndeye Marieme; Marcacci, Maurilia; Mbanne, Maïmouna; Ancora, Massimo; Secondini, Barbara; Di Lollo, Valeria; Teodori, Liana; Leone, Alessandra; Puglia, Ilaria; Gaye, Alioune; Sall, Amadou Alpha; Loucoubar, Cheikh; Rosà, Roberto; Diallo, Mawlouth; Monaco, Federica; Faye, Ousmane; Cammà, Cesare; Rizzoli, Annapaola; Savini, Giovanni; Faye, Oumar. - In: VIRUSES. - ISSN 1999-4915. - 15:6(2023), pp. 126101-126115. [10.3390/v15061261]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/379990
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