Astrocytes coordinate several homeostatic processes of the central nervous system and play essential roles for normal brain development and response to disease conditions. Protocols for the conversion of human induced pluripotent stem cells (hiPSCs) into mature astrocytes have opened to the generation of in vitro systems to explore astrocytes’ functions in living human cell contexts and patient-specific settings. In this study, we present an optimized monolayer procedure to commit hiPSC-derived cortical progenitors into enriched populations of cortical astrocyte progenitor cells (CX APCs) that can be further amplified and efficiently differentiated into mature astrocytes. Our optimized system provides a valid tool to explore the role of these cells in neurodevelopmental and neuropsychiatric diseases, opening it up to applications in drug development and biomarkers discovery/validation.

Cortical Astrocyte Progenitors and Astrocytes from Human Pluripotent Stem Cells / Battistella, Ingrid; Cutarelli, Alessandro; Zasso, Jacopo; Clerici, Massimo; Sala, Carlo; Marcatili, Matteo; Conti, Luciano. - In: JOURNAL OF PERSONALIZED MEDICINE. - ISSN 2075-4426. - 13:3(2023), pp. 53801-53813. [10.3390/jpm13030538]

Cortical Astrocyte Progenitors and Astrocytes from Human Pluripotent Stem Cells

Battistella, Ingrid
Co-primo
;
Cutarelli, Alessandro
Co-primo
;
Zasso, Jacopo
Secondo
;
Conti, Luciano
Ultimo
2023-01-01

Abstract

Astrocytes coordinate several homeostatic processes of the central nervous system and play essential roles for normal brain development and response to disease conditions. Protocols for the conversion of human induced pluripotent stem cells (hiPSCs) into mature astrocytes have opened to the generation of in vitro systems to explore astrocytes’ functions in living human cell contexts and patient-specific settings. In this study, we present an optimized monolayer procedure to commit hiPSC-derived cortical progenitors into enriched populations of cortical astrocyte progenitor cells (CX APCs) that can be further amplified and efficiently differentiated into mature astrocytes. Our optimized system provides a valid tool to explore the role of these cells in neurodevelopmental and neuropsychiatric diseases, opening it up to applications in drug development and biomarkers discovery/validation.
2023
3
Battistella, Ingrid; Cutarelli, Alessandro; Zasso, Jacopo; Clerici, Massimo; Sala, Carlo; Marcatili, Matteo; Conti, Luciano
Cortical Astrocyte Progenitors and Astrocytes from Human Pluripotent Stem Cells / Battistella, Ingrid; Cutarelli, Alessandro; Zasso, Jacopo; Clerici, Massimo; Sala, Carlo; Marcatili, Matteo; Conti, Luciano. - In: JOURNAL OF PERSONALIZED MEDICINE. - ISSN 2075-4426. - 13:3(2023), pp. 53801-53813. [10.3390/jpm13030538]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/373388
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