Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light, which makes this material applicable in additive manufacturing techniques and allows the prototyping and development of patient-specific 2D or 3D constructs. In this study, we produced a thin grid structure based on crosslinked Sil-MA that can be withdrawn and ejected and that can recover its shape after rehydration. A complete chemical and physical characterization of Sil-MA was first conducted. Additionally, we tested Sil-MA biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility of using it in future trials. Sil-MA was also tested to verify its ability to support osteogenesis. Overall, Sil-MA was shown to be biocompatible and osteoconductive. Finally, two different additive manufacturing technologies, a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique, were used to develop a Sil-MA grid construct. A proof-of-concept of its shape-memory property was provided. Together, our data support the hypothesis that Sil-MA grid constructs can be injectable and applicable in bone regeneration applications.

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration / Bucciarelli, Alessio; Petretta, Mauro; Grigolo, Brunella; Gambari, Laura; Bossi, Alessandra Maria; Grassi, Francesco; Maniglio, Devid. - In: GELS. - ISSN 2310-2861. - 8:12(2022), pp. 833.1-833.22. [10.3390/gels8120833]

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli, Alessio
Primo
;
Maniglio, Devid
Ultimo
2022-01-01

Abstract

Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light, which makes this material applicable in additive manufacturing techniques and allows the prototyping and development of patient-specific 2D or 3D constructs. In this study, we produced a thin grid structure based on crosslinked Sil-MA that can be withdrawn and ejected and that can recover its shape after rehydration. A complete chemical and physical characterization of Sil-MA was first conducted. Additionally, we tested Sil-MA biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility of using it in future trials. Sil-MA was also tested to verify its ability to support osteogenesis. Overall, Sil-MA was shown to be biocompatible and osteoconductive. Finally, two different additive manufacturing technologies, a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique, were used to develop a Sil-MA grid construct. A proof-of-concept of its shape-memory property was provided. Together, our data support the hypothesis that Sil-MA grid constructs can be injectable and applicable in bone regeneration applications.
2022
12
Bucciarelli, Alessio; Petretta, Mauro; Grigolo, Brunella; Gambari, Laura; Bossi, Alessandra Maria; Grassi, Francesco; Maniglio, Devid
Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration / Bucciarelli, Alessio; Petretta, Mauro; Grigolo, Brunella; Gambari, Laura; Bossi, Alessandra Maria; Grassi, Francesco; Maniglio, Devid. - In: GELS. - ISSN 2310-2861. - 8:12(2022), pp. 833.1-833.22. [10.3390/gels8120833]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/363043
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