TFE-fusion renal cell carcinomas (TFE-fusion RCCs) are caused by chromosomal translocations that lead to overexpression of the TFEB and TFE3 genes (Kauffman et al., 2014). The mechanisms leading to kidney tumor development remain uncharacterized and effective therapies are yet to be identified. Hence, the need to model these diseases in an experimental animal system (Kauffman et at, 2014). Here, we show that kidney-specific TFEB overexpression in transgenic mice, resulted in renal clear cells, multi-layered basement membranes, severe cystic pathology, and ultimately papillary carcinomas with hepatic metastases. These features closely recapitulate those observed in both TFEB- and TFE3-mediated human kidney tumors. Analysis of kidney samples revealed transcriptional induction and enhanced signaling of the WNT beta-catenin pathway. WNT signaling inhibitors normalized the proliferation rate of primary kidney cells and significantly rescued the disease phenotype in vivo. These data shed new light on the mechanisms underlying TFE-fusion RCCs and suggest a possible therapeutic strategy based on the inhibition of the WNT pathway.

Modeling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling / Calcagni, A; Kors, L; Verschuren, E; De Cegli, R; Zampelli, N; Nusco, E; Confalonieri, S; Bertalot, G; Pece, S; Settembre, C; Malouf, Gg; Leemans, Jc; de Heer, E; Salvatore, M; Peters, Djm; Di Fiore, Pp; Ballabio, A. - In: ELIFE. - ISSN 2050-084X. - 5:(2016), pp. e1704701-e1704726. [10.7554/eLife.17047]

Modeling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling

Bertalot G;
2016-01-01

Abstract

TFE-fusion renal cell carcinomas (TFE-fusion RCCs) are caused by chromosomal translocations that lead to overexpression of the TFEB and TFE3 genes (Kauffman et al., 2014). The mechanisms leading to kidney tumor development remain uncharacterized and effective therapies are yet to be identified. Hence, the need to model these diseases in an experimental animal system (Kauffman et at, 2014). Here, we show that kidney-specific TFEB overexpression in transgenic mice, resulted in renal clear cells, multi-layered basement membranes, severe cystic pathology, and ultimately papillary carcinomas with hepatic metastases. These features closely recapitulate those observed in both TFEB- and TFE3-mediated human kidney tumors. Analysis of kidney samples revealed transcriptional induction and enhanced signaling of the WNT beta-catenin pathway. WNT signaling inhibitors normalized the proliferation rate of primary kidney cells and significantly rescued the disease phenotype in vivo. These data shed new light on the mechanisms underlying TFE-fusion RCCs and suggest a possible therapeutic strategy based on the inhibition of the WNT pathway.
2016
Calcagni, A; Kors, L; Verschuren, E; De Cegli, R; Zampelli, N; Nusco, E; Confalonieri, S; Bertalot, G; Pece, S; Settembre, C; Malouf, Gg; Leemans, Jc; de Heer, E; Salvatore, M; Peters, Djm; Di Fiore, Pp; Ballabio, A
Modeling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling / Calcagni, A; Kors, L; Verschuren, E; De Cegli, R; Zampelli, N; Nusco, E; Confalonieri, S; Bertalot, G; Pece, S; Settembre, C; Malouf, Gg; Leemans, Jc; de Heer, E; Salvatore, M; Peters, Djm; Di Fiore, Pp; Ballabio, A. - In: ELIFE. - ISSN 2050-084X. - 5:(2016), pp. e1704701-e1704726. [10.7554/eLife.17047]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/361842
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