It is unclear whether the establishment of apical–basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and podocalyxin. Molecularly, IRSp53 acts by regulating the localization and activity of the small GTPase RAB35, and by interacting with the actin capping protein EPS8. Using correlative light and electron microscopy, we further show that IRSp53 ensures the shape and continuity of the opposing plasma membrane of two daughter cells, leading to the formation of a single apical lumen. Genetic removal of IRSp53 results in abnormal renal tubulogenesis, with altered tubular polarity and architectural organization. Thus, IRSp53 acts as a membrane curvature-sensing platform for the assembly of multi-protein complexes that control the trafficking of apical determinants and the integrity of the luminal plasma membrane.

IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules / Bisi, Sara; Marchesi, Stefano; Rizvi, Abrar; Carra, Davide; Beznoussenko, Galina; Ferrara, Ines; Deflorian, Gianluca; Mironov, Alexandre; Bertalot, Giovanni; Pisati, Federica; Oldani, Amanda; Cattaneo, Angela; Saberamoli, Ghazaleh; Pece, Salvatore; Viale, Giuseppe; Bachi, Angela; Claudio Tripodo, Claudio; Scita, Giorgio; Disanza, Andrea. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - STAMPA. - 11:1(2020), pp. 351601-351623. [10.1038/s41467-020-17091-x]

IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules

Bertalot, Giovanni;
2020-01-01

Abstract

It is unclear whether the establishment of apical–basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and podocalyxin. Molecularly, IRSp53 acts by regulating the localization and activity of the small GTPase RAB35, and by interacting with the actin capping protein EPS8. Using correlative light and electron microscopy, we further show that IRSp53 ensures the shape and continuity of the opposing plasma membrane of two daughter cells, leading to the formation of a single apical lumen. Genetic removal of IRSp53 results in abnormal renal tubulogenesis, with altered tubular polarity and architectural organization. Thus, IRSp53 acts as a membrane curvature-sensing platform for the assembly of multi-protein complexes that control the trafficking of apical determinants and the integrity of the luminal plasma membrane.
2020
1
Bisi, Sara; Marchesi, Stefano; Rizvi, Abrar; Carra, Davide; Beznoussenko, Galina; Ferrara, Ines; Deflorian, Gianluca; Mironov, Alexandre; Bertalot, Giovanni; Pisati, Federica; Oldani, Amanda; Cattaneo, Angela; Saberamoli, Ghazaleh; Pece, Salvatore; Viale, Giuseppe; Bachi, Angela; Claudio Tripodo, Claudio; Scita, Giorgio; Disanza, Andrea
IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules / Bisi, Sara; Marchesi, Stefano; Rizvi, Abrar; Carra, Davide; Beznoussenko, Galina; Ferrara, Ines; Deflorian, Gianluca; Mironov, Alexandre; Bertalot, Giovanni; Pisati, Federica; Oldani, Amanda; Cattaneo, Angela; Saberamoli, Ghazaleh; Pece, Salvatore; Viale, Giuseppe; Bachi, Angela; Claudio Tripodo, Claudio; Scita, Giorgio; Disanza, Andrea. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - STAMPA. - 11:1(2020), pp. 351601-351623. [10.1038/s41467-020-17091-x]
File in questo prodotto:
File Dimensione Formato  
Bisi.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 8.17 MB
Formato Adobe PDF
8.17 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/361804
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 20
social impact