Mammalian embryogenesis is a paradigm of regulative development as mouse embryos show plasticity in the regulation of cell fate, cell number, and tissue morphogenesis. However, the mechanisms behind embryo plasticity remain largely unknown. Here, we determine how mouse embryos respond to an increase in cell numbers to regulate the timing and mechanism of embryonic morphogenesis, leading to the formation of the pro-amniotic cavity. Using embryos and embryonic stem cell aggregates of different size, we show that while pro- amniotic cavity formation in normal-sized embryos is achieved through basement membrane-induced polarization and exocytosis, cavity formation of increased-size embryos is delayed and achieved through apoptosis of cells that lack contact with the basement mem- brane. Importantly, blocking apoptosis, both genetically and pharmacologically, alters pro-amniotic cavity formation but does not affect size regulation in enlarged embryos. We conclude that the regulation of embryonic size and morphogenesis, albeit concomitant, have distinct molecular underpinnings.

Embryo size regulates the timing and mechanism of pluripotent tissue morphogenesis / Orietti, Lorenzo C.; Souza Rosa, Viviane; Antonica, Francesco; Kyprianou, Christos; Mansfield, William; Marques-Souza, Henrique; Shahbazi, Marta N.; Zernicka-Goetz, Magdalena. - In: STEM CELL REPORTS. - ISSN 2213-6711. - 16:5(2021), pp. 1182-1196. [10.1016/j.stemcr.2020.09.004]

Embryo size regulates the timing and mechanism of pluripotent tissue morphogenesis

Francesco Antonica;
2021-01-01

Abstract

Mammalian embryogenesis is a paradigm of regulative development as mouse embryos show plasticity in the regulation of cell fate, cell number, and tissue morphogenesis. However, the mechanisms behind embryo plasticity remain largely unknown. Here, we determine how mouse embryos respond to an increase in cell numbers to regulate the timing and mechanism of embryonic morphogenesis, leading to the formation of the pro-amniotic cavity. Using embryos and embryonic stem cell aggregates of different size, we show that while pro- amniotic cavity formation in normal-sized embryos is achieved through basement membrane-induced polarization and exocytosis, cavity formation of increased-size embryos is delayed and achieved through apoptosis of cells that lack contact with the basement mem- brane. Importantly, blocking apoptosis, both genetically and pharmacologically, alters pro-amniotic cavity formation but does not affect size regulation in enlarged embryos. We conclude that the regulation of embryonic size and morphogenesis, albeit concomitant, have distinct molecular underpinnings.
2021
5
Orietti, Lorenzo C.; Souza Rosa, Viviane; Antonica, Francesco; Kyprianou, Christos; Mansfield, William; Marques-Souza, Henrique; Shahbazi, Marta N.; Zernicka-Goetz, Magdalena
Embryo size regulates the timing and mechanism of pluripotent tissue morphogenesis / Orietti, Lorenzo C.; Souza Rosa, Viviane; Antonica, Francesco; Kyprianou, Christos; Mansfield, William; Marques-Souza, Henrique; Shahbazi, Marta N.; Zernicka-Goetz, Magdalena. - In: STEM CELL REPORTS. - ISSN 2213-6711. - 16:5(2021), pp. 1182-1196. [10.1016/j.stemcr.2020.09.004]
File in questo prodotto:
File Dimensione Formato  
Embryo Size Regulates the Timing and Mechanism of Pluripotent Tissue Morphogenesis.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 2.87 MB
Formato Adobe PDF
2.87 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/352843
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 13
social impact