Scope: Epidemiological evidence suggests that a reduced risk of colorectal cancer (CRC) is correlated with high consumption of fruits and vegetables, which are major sources of fiber and phytochemicals, such as flavan-3-ols. However, it remains unknown how these phytochemicals and their specific gut-related metabolites may alter cancer cell behavior. Methods and results: A focused screening using native (poly)phenols and gut microbial metabolites (GMMs) on 3D HCT116 spheroids was carried out using a high-throughput imaging approach. Dose-responses, IC50, and long-term exposure were calculated for the most promising native (poly)phenols and GMMs. As a result, this research shows that (poly)phenol catabolites may play a key role in preventing cancer propagation. Indeed, μM concentration levels of (4R)-5-(3',4'-dihydroxyphenyl)-γ-valerolactone significantly decreased spheroid size at early stages of spheroid aggregation and gene expression of matrix metalloproteinases. Conclusion: A chronic exposure to (4R)-5-(3',4'-dihydroxyphenyl)-γ-valerolactone may lead to a reduced CRC risk. Daily intake of monomeric, oligomeric, and polymeric flavan-3-ols might increase the colonic concentrations of this metabolite, and, in turn, this compound may act locally interacting with intestinal epithelial cells, precancerous and cancer cells. This article is protected by copyright. All rights reserved.

A Screening of Native (Poly)phenols and Gut-Related Metabolites on 3D HCT116 Spheroids Reveals Gut Health Benefits of a Flavan-3-ol Metabolite / Rubert, Josep; Gatto, Pamela; Pancher, Michael; Sidarovich, Viktoryia; Curti, Claudio; Mena, Pedro; Rio, Daniele Del; Quattrone, Alessandro; Mattivi, Fulvio. - In: MOLECULAR NUTRITION & FOOD RESEARCH. - ISSN 1613-4125. - 2022:(2022), pp. e210104301-e210104310. [10.1002/mnfr.202101043]

A Screening of Native (Poly)phenols and Gut-Related Metabolites on 3D HCT116 Spheroids Reveals Gut Health Benefits of a Flavan-3-ol Metabolite

Rubert, Josep;Gatto, Pamela;Pancher, Michael;Sidarovich, Viktoryia;Quattrone, Alessandro;Mattivi, Fulvio
2022

Abstract

Scope: Epidemiological evidence suggests that a reduced risk of colorectal cancer (CRC) is correlated with high consumption of fruits and vegetables, which are major sources of fiber and phytochemicals, such as flavan-3-ols. However, it remains unknown how these phytochemicals and their specific gut-related metabolites may alter cancer cell behavior. Methods and results: A focused screening using native (poly)phenols and gut microbial metabolites (GMMs) on 3D HCT116 spheroids was carried out using a high-throughput imaging approach. Dose-responses, IC50, and long-term exposure were calculated for the most promising native (poly)phenols and GMMs. As a result, this research shows that (poly)phenol catabolites may play a key role in preventing cancer propagation. Indeed, μM concentration levels of (4R)-5-(3',4'-dihydroxyphenyl)-γ-valerolactone significantly decreased spheroid size at early stages of spheroid aggregation and gene expression of matrix metalloproteinases. Conclusion: A chronic exposure to (4R)-5-(3',4'-dihydroxyphenyl)-γ-valerolactone may lead to a reduced CRC risk. Daily intake of monomeric, oligomeric, and polymeric flavan-3-ols might increase the colonic concentrations of this metabolite, and, in turn, this compound may act locally interacting with intestinal epithelial cells, precancerous and cancer cells. This article is protected by copyright. All rights reserved.
Rubert, Josep; Gatto, Pamela; Pancher, Michael; Sidarovich, Viktoryia; Curti, Claudio; Mena, Pedro; Rio, Daniele Del; Quattrone, Alessandro; Mattivi, Fulvio
A Screening of Native (Poly)phenols and Gut-Related Metabolites on 3D HCT116 Spheroids Reveals Gut Health Benefits of a Flavan-3-ol Metabolite / Rubert, Josep; Gatto, Pamela; Pancher, Michael; Sidarovich, Viktoryia; Curti, Claudio; Mena, Pedro; Rio, Daniele Del; Quattrone, Alessandro; Mattivi, Fulvio. - In: MOLECULAR NUTRITION & FOOD RESEARCH. - ISSN 1613-4125. - 2022:(2022), pp. e210104301-e210104310. [10.1002/mnfr.202101043]
File in questo prodotto:
File Dimensione Formato  
Rubert_et_al_Molecular Nutrition Food Res - 2022 A Screening of Native Poly phenols and Gut‐Related Metabolites on 3D HCT116.pdf

accesso aperto

Descrizione: articolo principale, versione editoriale
Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 1.18 MB
Formato Adobe PDF
1.18 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/339932
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact