Double-stranded RNAs (dsRNAs) are abundantly present in cells, playing multiple regulatory functions. dsRNAs of viral origin activate innate immune responses. Since RNA editing and modifications affect the structure and recognition of RNAs, their alteration can result in the accumulation of aberrant endogenous dsRNAs inducing a deleterious innate immune response. Here, we present a complete protocol for the measurement of dsRNAs in a live mouse tissue using dsRNA immunoprecipitation and sequencing (dsRIP-Seq). This protocol focuses on tissue isolation, dsRNA immunoprecipitation and downstream computational analysis. For complete details on the use and execution of this protocol, please refer to Gao et al. (2020).

Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq / Gao, Y; Chen, S; Halene, S; Tebaldi, T. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 2:1(2021), pp. 10036601-10036612. [10.1016/j.xpro.2021.100366]

Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq

Tebaldi T
2021-01-01

Abstract

Double-stranded RNAs (dsRNAs) are abundantly present in cells, playing multiple regulatory functions. dsRNAs of viral origin activate innate immune responses. Since RNA editing and modifications affect the structure and recognition of RNAs, their alteration can result in the accumulation of aberrant endogenous dsRNAs inducing a deleterious innate immune response. Here, we present a complete protocol for the measurement of dsRNAs in a live mouse tissue using dsRNA immunoprecipitation and sequencing (dsRIP-Seq). This protocol focuses on tissue isolation, dsRNA immunoprecipitation and downstream computational analysis. For complete details on the use and execution of this protocol, please refer to Gao et al. (2020).
2021
1
Gao, Y; Chen, S; Halene, S; Tebaldi, T
Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq / Gao, Y; Chen, S; Halene, S; Tebaldi, T. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 2:1(2021), pp. 10036601-10036612. [10.1016/j.xpro.2021.100366]
File in questo prodotto:
File Dimensione Formato  
2021_Gao_Tebaldi_STAR_protocols.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 2.87 MB
Formato Adobe PDF
2.87 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/327996
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 4
  • ???jsp.display-item.citation.isi??? ND
social impact