The neuroscience field has increased enormously over the last decades, achieving the possible real application of neuronal cultures not only for reproducing neural architectures resembling in vivo tissues, but also for the development of functional devices. In this context, surface patterning for cell confinement is crucial, and new active materials together with new protocols for preparing substrates suitable for confining cells, guiding their processes in the desired configuration are extremely appreciated. Here, TiO2 sol-gel derived films were selected as proof-of-concept materials to grow neurons in suitable confined configurations, taking advantage of the biocompatible properties of modified TiO2 substrates. TiO2 sol-gel derived films were made compatible with the growth of neurons thanks to a stable and controlled poly-lysine coating, obtained by silanization chemistry and streptavidin-biotin interactions. Moreover, a spotting protocol, here described and optimized, allowed the simple preparation of arrays of neurons, where cell adhesion was guided in specific areas and the neurites development driven in the desired arrangement. The resulting arrays were successfully tested for the growth and differentiation of neurons, demonstrating the possible adhesion of cells in specific areas of the film, therefore paving the way to applications such as the direct growth of excitable cells nearby electrodes of devices, with an evident enhancement of cell-electrodes communication.

Functionalization of TiO2 sol-gel derived films for cell confinement / Pasquardini, L.; Roncador, A.; Prusakova, V.; Vanzetti, L.; Potrich, C.; Lunelli, L.; Pederzolli, C.; Iannotta, S.; Macchi, P.; Dirè, S.. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - 204:(2021), pp. 111787.1-111787.8. [10.1016/j.colsurfb.2021.111787]

Functionalization of TiO2 sol-gel derived films for cell confinement

Pasquardini L.;Roncador A.;Prusakova V.;Lunelli L.;Macchi P.;Dirè S.
2021

Abstract

The neuroscience field has increased enormously over the last decades, achieving the possible real application of neuronal cultures not only for reproducing neural architectures resembling in vivo tissues, but also for the development of functional devices. In this context, surface patterning for cell confinement is crucial, and new active materials together with new protocols for preparing substrates suitable for confining cells, guiding their processes in the desired configuration are extremely appreciated. Here, TiO2 sol-gel derived films were selected as proof-of-concept materials to grow neurons in suitable confined configurations, taking advantage of the biocompatible properties of modified TiO2 substrates. TiO2 sol-gel derived films were made compatible with the growth of neurons thanks to a stable and controlled poly-lysine coating, obtained by silanization chemistry and streptavidin-biotin interactions. Moreover, a spotting protocol, here described and optimized, allowed the simple preparation of arrays of neurons, where cell adhesion was guided in specific areas and the neurites development driven in the desired arrangement. The resulting arrays were successfully tested for the growth and differentiation of neurons, demonstrating the possible adhesion of cells in specific areas of the film, therefore paving the way to applications such as the direct growth of excitable cells nearby electrodes of devices, with an evident enhancement of cell-electrodes communication.
Pasquardini, L.; Roncador, A.; Prusakova, V.; Vanzetti, L.; Potrich, C.; Lunelli, L.; Pederzolli, C.; Iannotta, S.; Macchi, P.; Dirè, S.
Functionalization of TiO2 sol-gel derived films for cell confinement / Pasquardini, L.; Roncador, A.; Prusakova, V.; Vanzetti, L.; Potrich, C.; Lunelli, L.; Pederzolli, C.; Iannotta, S.; Macchi, P.; Dirè, S.. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - 204:(2021), pp. 111787.1-111787.8. [10.1016/j.colsurfb.2021.111787]
File in questo prodotto:
File Dimensione Formato  
2021_Colloids.pdf

Solo gestori archivio

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 5.59 MB
Formato Adobe PDF
5.59 MB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/314651
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact