Single-cell analysis techniques are fundamental to study the heterogeneity of cellular populations, which is the basis to understand several biomedical mechanisms. Light-sheet fluorescence microscopy is a powerful technique for obtaining high-resolution imaging of individual cells, but the complexity of the setup and the sample mounting procedures limit its overall throughput. In our work, we present an optofluidic microscope-on-chip with integrated microlenses for light-sheet shaping and with a fluidic microchannel that allows the automatic and continuous delivery of samples of a few tens of microns in size. The device is used to perform dual-color fluorescence analysis and 3D reconstruction of xenograft-derived mouse breast cancer cells.

High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip / Sala, F.; Castriotta, M.; Paie, P.; Farina, A.; D'Annunzio, S.; Zippo, A.; Osellame, R.; Bragheri, F.; Bassi, A.. - In: BIOMEDICAL OPTICS EXPRESS. - ISSN 2156-7085. - 11:8(2020), pp. 4397-4407. [10.1364/BOE.393892]

High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip

Farina A.;D'Annunzio S.;Zippo A.;
2020

Abstract

Single-cell analysis techniques are fundamental to study the heterogeneity of cellular populations, which is the basis to understand several biomedical mechanisms. Light-sheet fluorescence microscopy is a powerful technique for obtaining high-resolution imaging of individual cells, but the complexity of the setup and the sample mounting procedures limit its overall throughput. In our work, we present an optofluidic microscope-on-chip with integrated microlenses for light-sheet shaping and with a fluidic microchannel that allows the automatic and continuous delivery of samples of a few tens of microns in size. The device is used to perform dual-color fluorescence analysis and 3D reconstruction of xenograft-derived mouse breast cancer cells.
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Sala, F.; Castriotta, M.; Paie, P.; Farina, A.; D'Annunzio, S.; Zippo, A.; Osellame, R.; Bragheri, F.; Bassi, A.
High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip / Sala, F.; Castriotta, M.; Paie, P.; Farina, A.; D'Annunzio, S.; Zippo, A.; Osellame, R.; Bragheri, F.; Bassi, A.. - In: BIOMEDICAL OPTICS EXPRESS. - ISSN 2156-7085. - 11:8(2020), pp. 4397-4407. [10.1364/BOE.393892]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/287535
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