Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.
Repo-Man/PP1 regulates heterochromatin formation in interphase / De Castro, I.J., Budzak, J., Di Giacinto, M.L., Ligammari, L., Gokhan, E., Spanos, C., Moralli, D., Richardson, C., De Las Heras, J.I., Salatino, S., Schirmer, E.C., Ullman, K.S., Bickmore, W.A., Green, C., Rappsilber, J., Lamble, S., Goldberg, M.W., Vinciotti, V., Vagnarelli, P.. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 8:1(2017), p. 14048. [10.1038/ncomms14048]
Repo-Man/PP1 regulates heterochromatin formation in interphase
Vinciotti V.;
2017-01-01
Abstract
Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione
