Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.
Repo-Man/PP1 regulates heterochromatin formation in interphase / De Castro, I. J.; Budzak, J.; Di Giacinto, M. L.; Ligammari, L.; Gokhan, E.; Spanos, C.; Moralli, D.; Richardson, C.; De Las Heras, J. I.; Salatino, S.; Schirmer, E. C.; Ullman, K. S.; Bickmore, W. A.; Green, C.; Rappsilber, J.; Lamble, S.; Goldberg, M. W.; Vinciotti, V.; Vagnarelli, P.. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 8:1(2017), p. 14048. [10.1038/ncomms14048]
Repo-Man/PP1 regulates heterochromatin formation in interphase
Vinciotti V.;
2017-01-01
Abstract
Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione
