Real-Time Behavior of a Microalgae–Bacteria Consortium Treating Wastewater in a Sequencing Batch Reactor in Response to Feeding Time and Agitation Mode

A study of a microalgae–bacteria treatment system was conducted in a sequencing batch reactor (SBR) by combining a precultured native algae Nannochloropsis gaditana L2 with spontaneous municipal wastewater microorganisms. Two types of agitation, air mixing (AI) and mechanical mixing (MIX), were assessed at continuous illumination (L) and photoperiod cycle light/dark (L/D). The obtained consortium, via native microalgae addition, has a better operational efficiency compared to spontaneous control. This allows the removal of 78% and 53% of total Kjeldhal nitrogen (TKN) and chemical oxygen demand (COD), respectively. Under the (L/D) photoperiod, the optimal removal rate (90% of TKN and 75% of COD) was obtained by the consortium at 4 days of hydraulic retention time (HRT) using the AI mode. Moreover, during feeding during dark (D/L) photoperiod, the highest removal rate (83% TKN and 82% COD) was recorded at 4 days HRT using the AI mode. These results bring, at the scale of a bioreactor, new data regarding the mode of aeration and the feeding time. They prove the concept of such a technology, increasing the attraction of microalgae-based wastewater treatment.