The purpose of this study is to produce injectable taurine (Tr)-loaded alginate (Agn) hydrogel for age-related macular degeneration (AMD) treatment by inducing the regeneration of RPE (retinal pigment epithelium) cells. Porosity and swelling ratio were measured to evaluate the mechanical properties of the hydrogels, and Fourier transform infrared spectroscopy (FTIR) was used to evaluate the physical and chemical properties. RPE cells extracted from the pigmented epithelium of rabbits were encapsulated in the Tr/Agn hydrogels. Cells proliferation and migration were improved in Tr/Agn hydrogels with an enhanced expression of RPE-specific genes including RPE65, CRALBP, NPR-A, MITF and collagen type I and II. In vivo tests demonstrated the excellent biocompatibility and biodegradability without inflammatory response by the host when implanted with the hydrogel. Moreover, when the Tr/Agn hydrogels were injected into the sub-retinal space, high adhesion of RPE cells and retinal regeneration were confirmed. These results demonstrated a potential role of injectable Tr/Agn hydrogels as potential therapeutic tools for the treatment of retinal diseases, including AMD.

Injectable taurine-loaded alginate hydrogels for retinal pigment epithelium (RPE) regeneration / Shin, E. Y.; Park, J. H.; Shin, M. E.; Song, J. E.; Thangavelu, M.; Carlomagno, C.; Motta, A.; Migliaresi, C.; Khang, G.. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - ELETTRONICO. - 103:(2019), p. 109787. [10.1016/j.msec.2019.109787]

Injectable taurine-loaded alginate hydrogels for retinal pigment epithelium (RPE) regeneration

Shin M. E.;Carlomagno C.;Motta A.;Migliaresi C.;Khang G.
2019-01-01

Abstract

The purpose of this study is to produce injectable taurine (Tr)-loaded alginate (Agn) hydrogel for age-related macular degeneration (AMD) treatment by inducing the regeneration of RPE (retinal pigment epithelium) cells. Porosity and swelling ratio were measured to evaluate the mechanical properties of the hydrogels, and Fourier transform infrared spectroscopy (FTIR) was used to evaluate the physical and chemical properties. RPE cells extracted from the pigmented epithelium of rabbits were encapsulated in the Tr/Agn hydrogels. Cells proliferation and migration were improved in Tr/Agn hydrogels with an enhanced expression of RPE-specific genes including RPE65, CRALBP, NPR-A, MITF and collagen type I and II. In vivo tests demonstrated the excellent biocompatibility and biodegradability without inflammatory response by the host when implanted with the hydrogel. Moreover, when the Tr/Agn hydrogels were injected into the sub-retinal space, high adhesion of RPE cells and retinal regeneration were confirmed. These results demonstrated a potential role of injectable Tr/Agn hydrogels as potential therapeutic tools for the treatment of retinal diseases, including AMD.
2019
Shin, E. Y.; Park, J. H.; Shin, M. E.; Song, J. E.; Thangavelu, M.; Carlomagno, C.; Motta, A.; Migliaresi, C.; Khang, G.
Injectable taurine-loaded alginate hydrogels for retinal pigment epithelium (RPE) regeneration / Shin, E. Y.; Park, J. H.; Shin, M. E.; Song, J. E.; Thangavelu, M.; Carlomagno, C.; Motta, A.; Migliaresi, C.; Khang, G.. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - ELETTRONICO. - 103:(2019), p. 109787. [10.1016/j.msec.2019.109787]
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