Allele-specific expression arises when transcriptional activity at the different alleles of a gene differs considerably. Although extensive research has been carried out to detect and characterize this phenomenon, the landscape of allele-specific expression in cancer is still poorly understood. In this chapter, we describe a fast and reliable analysis pipeline to study allele-specific expression in cancer using next-generation sequencing data. The pipeline provides a gene-level analysis approach that exploits paired germline DNA and tumor RNA sequencing data and benefits from parallel computation resources when available.

Allele-specific expression analysis in cancer using next-generation sequencing data / Romanel, A.. - STAMPA. - 1878:(2019), pp. 125-137. [10.1007/978-1-4939-8868-6_7]

Allele-specific expression analysis in cancer using next-generation sequencing data

Romanel A.
2019-01-01

Abstract

Allele-specific expression arises when transcriptional activity at the different alleles of a gene differs considerably. Although extensive research has been carried out to detect and characterize this phenomenon, the landscape of allele-specific expression in cancer is still poorly understood. In this chapter, we describe a fast and reliable analysis pipeline to study allele-specific expression in cancer using next-generation sequencing data. The pipeline provides a gene-level analysis approach that exploits paired germline DNA and tumor RNA sequencing data and benefits from parallel computation resources when available.
2019
Methods in Molecular Biology
USA
Humana Press Inc.
978-1-4939-8866-2
978-1-4939-8868-6
Romanel, A.
Allele-specific expression analysis in cancer using next-generation sequencing data / Romanel, A.. - STAMPA. - 1878:(2019), pp. 125-137. [10.1007/978-1-4939-8868-6_7]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/257910
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