Allele-specific expression arises when transcriptional activity at the different alleles of a gene differs considerably. Although extensive research has been carried out to detect and characterize this phenomenon, the landscape of allele-specific expression in cancer is still poorly understood. In this chapter, we describe a fast and reliable analysis pipeline to study allele-specific expression in cancer using next-generation sequencing data. The pipeline provides a gene-level analysis approach that exploits paired germline DNA and tumor RNA sequencing data and benefits from parallel computation resources when available.
Allele-specific expression analysis in cancer using next-generation sequencing data / Romanel, A.. - STAMPA. - 1878:(2019), pp. 125-137. [10.1007/978-1-4939-8868-6_7]
Allele-specific expression analysis in cancer using next-generation sequencing data
Romanel A.
2019-01-01
Abstract
Allele-specific expression arises when transcriptional activity at the different alleles of a gene differs considerably. Although extensive research has been carried out to detect and characterize this phenomenon, the landscape of allele-specific expression in cancer is still poorly understood. In this chapter, we describe a fast and reliable analysis pipeline to study allele-specific expression in cancer using next-generation sequencing data. The pipeline provides a gene-level analysis approach that exploits paired germline DNA and tumor RNA sequencing data and benefits from parallel computation resources when available.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione