Grapevine root rot, caused by Armillaria mellea, is a seri-ous disease in some grape-growing regions. Young grape-vines start to show symptoms of Armillaria root rot from the second year after inoculation, suggesting a certain de-gree of resistance in young roots. We used a suppression subtractive hybridization approach to study grapevine's reactions to the first stages of A. mellea infection. We iden-tified 24 genes that were upregulated in the roots of the rootstock Kober 5BB 24 h after A. mellea challenge. Real-time reverse-transcriptase polymerase chain reaction analy-sis confirmed the induction of genes encoding protease inhibitors, thaumatins, glutathione S-transferase, and ami-nocyclopropane carboxylate oxidase, as well as phase-change related, tumor-related, and proline-rich proteins, and gene markers of the ethylene and jasmonate signaling pathway. Gene modulation was generally stronger in Kober 5BB than in Pinot Noir plants, and in vitro inocula-tion induced higher modulation than in greenhouse Armillaria spp. treatments. The full-length coding sequences of seven of these genes were obtained and expressed as re-combinant proteins. The grapevine homologue of the Quer-cus spp. phase-change-related protein inhibited the growth of A. mellea mycelia in vitro, suggesting that this protein may play an important role in the defense response against A. mellea. © 2010 The American Phytopathological Society.
Armillaria mellea induces a set of defense genes in grapevine roots and one of them codifies a protein with antifungal activity / Perazzolli, M.; Bampi, F.; Faccin, S.; Moser, M.; De Luca, F.; Ciccotti, A. M.; Velasco, R.; Gessler, C.; Pertot, I.; Moser, C.. - In: MOLECULAR PLANT-MICROBE INTERACTIONS. - ISSN 0894-0282. - 23:4(2010), pp. 485-496. [10.1094/MPMI-23-4-0485]
Armillaria mellea induces a set of defense genes in grapevine roots and one of them codifies a protein with antifungal activity
Perazzolli M.;Pertot I.;
2010-01-01
Abstract
Grapevine root rot, caused by Armillaria mellea, is a seri-ous disease in some grape-growing regions. Young grape-vines start to show symptoms of Armillaria root rot from the second year after inoculation, suggesting a certain de-gree of resistance in young roots. We used a suppression subtractive hybridization approach to study grapevine's reactions to the first stages of A. mellea infection. We iden-tified 24 genes that were upregulated in the roots of the rootstock Kober 5BB 24 h after A. mellea challenge. Real-time reverse-transcriptase polymerase chain reaction analy-sis confirmed the induction of genes encoding protease inhibitors, thaumatins, glutathione S-transferase, and ami-nocyclopropane carboxylate oxidase, as well as phase-change related, tumor-related, and proline-rich proteins, and gene markers of the ethylene and jasmonate signaling pathway. Gene modulation was generally stronger in Kober 5BB than in Pinot Noir plants, and in vitro inocula-tion induced higher modulation than in greenhouse Armillaria spp. treatments. The full-length coding sequences of seven of these genes were obtained and expressed as re-combinant proteins. The grapevine homologue of the Quer-cus spp. phase-change-related protein inhibited the growth of A. mellea mycelia in vitro, suggesting that this protein may play an important role in the defense response against A. mellea. © 2010 The American Phytopathological Society.File | Dimensione | Formato | |
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Perazzolli et al 2010.pdf
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