Erysiphe necator is the causal agent of powdery mildew (PM), one of the most destructive diseases of grapevine. PM is controlled by sulfur-based and synthetic fungicides, which every year are dispersed into the environment. This is why PM-resistant varieties should become a priority for sustainable grapevine and wine production. PM resistance can be achieved in other crops by knocking out susceptibility S-genes, such as those residing at genetic loci known as MLO (Mildew Locus O). All MLO S-genes of dicots belong to the phylogenetic clade V, including grapevine genes VvMLO7, 11 and 13, which are upregulated during PM infection, and VvMLO6, which is not upregulated. Before adopting a gene-editing approach to knockout candidate S-genes, the evidence that loss of function of MLO genes can reduce PM susceptibility is necessary. This paper reports the knockdown through RNA interference of VvMLO6, 7, 11 and 13. The knockdown of VvMLO6, 11 and 13 did not decrease PM severity, whereas the knockdown of VvMLO7 in combination with VvMLO6 and VvMLO11 reduced PM severity up to 77%. The knockdown of VvMLO7 and VvMLO6 seemed to be important for PM resistance, whereas a role for VvMLO11 does not seem likely. Cell wall appositions (papillae) were present in both resistant and susceptible lines in response to PM attack. Thirteen genes involved in defense were less upregulated in infected mlo plants, highlighting the early mlo-dependent disruption of PM invasion

Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine / Pessina, Stefano; Lenzi, Luisa; Perazzolli, Michele; Campa, M.; Dalla Costa, Lorenza; Urso, S.; Vale, G.; Salamini, F.; Velasco, Riccardo; Malnoy, Mickael Arnaud. - In: HORTICULTURE RESEARCH. - ISSN 2052-7276. - 3:16016(2016), pp. 16016.1-16016.9. [10.1038/hortres.2016.16]

Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine

Perazzolli, Michele;Velasco, Riccardo;
2016

Abstract

Erysiphe necator is the causal agent of powdery mildew (PM), one of the most destructive diseases of grapevine. PM is controlled by sulfur-based and synthetic fungicides, which every year are dispersed into the environment. This is why PM-resistant varieties should become a priority for sustainable grapevine and wine production. PM resistance can be achieved in other crops by knocking out susceptibility S-genes, such as those residing at genetic loci known as MLO (Mildew Locus O). All MLO S-genes of dicots belong to the phylogenetic clade V, including grapevine genes VvMLO7, 11 and 13, which are upregulated during PM infection, and VvMLO6, which is not upregulated. Before adopting a gene-editing approach to knockout candidate S-genes, the evidence that loss of function of MLO genes can reduce PM susceptibility is necessary. This paper reports the knockdown through RNA interference of VvMLO6, 7, 11 and 13. The knockdown of VvMLO6, 11 and 13 did not decrease PM severity, whereas the knockdown of VvMLO7 in combination with VvMLO6 and VvMLO11 reduced PM severity up to 77%. The knockdown of VvMLO7 and VvMLO6 seemed to be important for PM resistance, whereas a role for VvMLO11 does not seem likely. Cell wall appositions (papillae) were present in both resistant and susceptible lines in response to PM attack. Thirteen genes involved in defense were less upregulated in infected mlo plants, highlighting the early mlo-dependent disruption of PM invasion
16016
Pessina, Stefano; Lenzi, Luisa; Perazzolli, Michele; Campa, M.; Dalla Costa, Lorenza; Urso, S.; Vale, G.; Salamini, F.; Velasco, Riccardo; Malnoy, Mickael Arnaud
Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine / Pessina, Stefano; Lenzi, Luisa; Perazzolli, Michele; Campa, M.; Dalla Costa, Lorenza; Urso, S.; Vale, G.; Salamini, F.; Velasco, Riccardo; Malnoy, Mickael Arnaud. - In: HORTICULTURE RESEARCH. - ISSN 2052-7276. - 3:16016(2016), pp. 16016.1-16016.9. [10.1038/hortres.2016.16]
File in questo prodotto:
File Dimensione Formato  
Pessina 2016 et al.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 2.42 MB
Formato Adobe PDF
2.42 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11572/255337
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 90
  • ???jsp.display-item.citation.isi??? 87
social impact