Dried and sterile acellular esophageal matrix was obtained within a new drying process based on the use of supercritical carbon dioxide (SC-CO2). Experiments were performed coupling a conventional detergent enzymatic treatment with two different drying methods: (i) SC-CO2 drying alone; (ii) dehydration in ethanol and a subsequent SC-CO2 drying. Long term preservation was achieved for several months after drying, demonstrating the maintenance of extracellular matrix (ECM) structure, mechanical properties and biocompatibility within cell repopulation studies in vitro. Overall, the results highlighted the potential of this novel technology to obtain a dry and sterile acellular matrix that can be easily stored for oesophageal regeneration in patients with emergency need.

Long-term maintenance of dried acellular matrices / Zambon, Alessandro; Giobbe, Giovanni; Vetralla, Massimo; Michelino, Filippo; Urbani, Luca; Pantano, Maria; Pugno, Nicola; De Coppi, Paolo; Elvassore, Nicola; Spilimbergo, Sara. - (2018), pp. 1091-1097. (Intervento presentato al convegno 21st International Drying Symposium tenutosi a Valencia, Spain nel 11-14 Settembre 2018) [10.4995/IDS2018.2018.7844].

Long-term maintenance of dried acellular matrices

Pantano, Maria;Pugno, Nicola;
2018-01-01

Abstract

Dried and sterile acellular esophageal matrix was obtained within a new drying process based on the use of supercritical carbon dioxide (SC-CO2). Experiments were performed coupling a conventional detergent enzymatic treatment with two different drying methods: (i) SC-CO2 drying alone; (ii) dehydration in ethanol and a subsequent SC-CO2 drying. Long term preservation was achieved for several months after drying, demonstrating the maintenance of extracellular matrix (ECM) structure, mechanical properties and biocompatibility within cell repopulation studies in vitro. Overall, the results highlighted the potential of this novel technology to obtain a dry and sterile acellular matrix that can be easily stored for oesophageal regeneration in patients with emergency need.
2018
21st International Drying Symposium
CAMINO VERA S-N, VALENCIA, 46022, SPAIN
UNIV POLITECNICA VALENCIA
9788490486887
Zambon, Alessandro; Giobbe, Giovanni; Vetralla, Massimo; Michelino, Filippo; Urbani, Luca; Pantano, Maria; Pugno, Nicola; De Coppi, Paolo; Elvassore, ...espandi
Long-term maintenance of dried acellular matrices / Zambon, Alessandro; Giobbe, Giovanni; Vetralla, Massimo; Michelino, Filippo; Urbani, Luca; Pantano, Maria; Pugno, Nicola; De Coppi, Paolo; Elvassore, Nicola; Spilimbergo, Sara. - (2018), pp. 1091-1097. (Intervento presentato al convegno 21st International Drying Symposium tenutosi a Valencia, Spain nel 11-14 Settembre 2018) [10.4995/IDS2018.2018.7844].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/242996
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