Skeletal muscle is composed of different myofiber types that preferentially use glucose or lipids for ATP production. How fuel preference is regulated in these post-mitotic cells is largely unknown, making this issue a key question in the fields of muscle and whole-body metabolism. Here, we show that microRNAs (miRNAs) play a role in defining myofiber metabolic profiles. mRNA and miRNA signatures of all myofiber types obtained at the single-cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated mouse myofiber type-specific catalog of gene and miRNA expression and establishes miR-27a-3p and miR-142-3p as regulators of lipid use in skeletal muscle. Chemello et al. characterize coding mRNAs and non-coding microRNAs expressed by myofibers of hindlimb mouse muscles, identifying complex interactions between these molecules that modulate mitochondrial functions and muscle metabolism. They demonstrate that specific short non-coding RNAs influence the contractile fiber composition of skeletal muscles by modulating muscle metabolism.

Transcriptomic Analysis of Single Isolated Myofibers Identifies miR-27a-3p and miR-142-3p as Regulators of Metabolism in Skeletal Muscle / Chemello, Francesco; Grespi, Francesca; Zulian, Alessandra; Cancellara, Pasqua; Hebert-Chatelain, Etienne; Martini, Paolo; Bean, Camilla; Alessio, Enrico; Buson, Lisa; Bazzega, Martina; Armani, Andrea; Sandri, Marco; Ferrazza, Ruggero; Laveder, Paolo; Guella, Graziano; Reggiani, Carlo; Romualdi, Chiara; Bernardi, Paolo; Scorrano, Luca; Cagnin, Stefano; Lanfranchi, Gerolamo. - In: CELL REPORTS. - ISSN 2211-1247. - 26:13(2019), pp. 3784-3797. [10.1016/j.celrep.2019.02.105]

Transcriptomic Analysis of Single Isolated Myofibers Identifies miR-27a-3p and miR-142-3p as Regulators of Metabolism in Skeletal Muscle

Ferrazza, Ruggero;Guella, Graziano;
2019-01-01

Abstract

Skeletal muscle is composed of different myofiber types that preferentially use glucose or lipids for ATP production. How fuel preference is regulated in these post-mitotic cells is largely unknown, making this issue a key question in the fields of muscle and whole-body metabolism. Here, we show that microRNAs (miRNAs) play a role in defining myofiber metabolic profiles. mRNA and miRNA signatures of all myofiber types obtained at the single-cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated mouse myofiber type-specific catalog of gene and miRNA expression and establishes miR-27a-3p and miR-142-3p as regulators of lipid use in skeletal muscle. Chemello et al. characterize coding mRNAs and non-coding microRNAs expressed by myofibers of hindlimb mouse muscles, identifying complex interactions between these molecules that modulate mitochondrial functions and muscle metabolism. They demonstrate that specific short non-coding RNAs influence the contractile fiber composition of skeletal muscles by modulating muscle metabolism.
2019
13
Chemello, Francesco; Grespi, Francesca; Zulian, Alessandra; Cancellara, Pasqua; Hebert-Chatelain, Etienne; Martini, Paolo; Bean, Camilla; Alessio, Enr...espandi
Transcriptomic Analysis of Single Isolated Myofibers Identifies miR-27a-3p and miR-142-3p as Regulators of Metabolism in Skeletal Muscle / Chemello, Francesco; Grespi, Francesca; Zulian, Alessandra; Cancellara, Pasqua; Hebert-Chatelain, Etienne; Martini, Paolo; Bean, Camilla; Alessio, Enrico; Buson, Lisa; Bazzega, Martina; Armani, Andrea; Sandri, Marco; Ferrazza, Ruggero; Laveder, Paolo; Guella, Graziano; Reggiani, Carlo; Romualdi, Chiara; Bernardi, Paolo; Scorrano, Luca; Cagnin, Stefano; Lanfranchi, Gerolamo. - In: CELL REPORTS. - ISSN 2211-1247. - 26:13(2019), pp. 3784-3797. [10.1016/j.celrep.2019.02.105]
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