Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.

Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2 / Jusuf, Patricia R.; Albadri, Shahad; Paolini, Alessio; Currie, Peter D.; Argenton, Francesco; Higashijima, Shin-ichi; Harris, William A.; Poggi, Lucia. - In: THE JOURNAL OF NEUROSCIENCE. - ISSN 0270-6474. - 2012, 32:40(2012), pp. 13929-13944. [10.1523/JNEUROSCI.2073-12.2012]

Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2

Argenton, Francesco;Poggi, Lucia
2012

Abstract

Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.
40
Jusuf, Patricia R.; Albadri, Shahad; Paolini, Alessio; Currie, Peter D.; Argenton, Francesco; Higashijima, Shin-ichi; Harris, William A.; Poggi, Lucia
Biasing amacrine subtypes in the Atoh7 lineage through expression of barhl2 / Jusuf, Patricia R.; Albadri, Shahad; Paolini, Alessio; Currie, Peter D.; Argenton, Francesco; Higashijima, Shin-ichi; Harris, William A.; Poggi, Lucia. - In: THE JOURNAL OF NEUROSCIENCE. - ISSN 0270-6474. - 2012, 32:40(2012), pp. 13929-13944. [10.1523/JNEUROSCI.2073-12.2012]
File in questo prodotto:
File Dimensione Formato  
JNS_2012.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 9.11 MB
Formato Adobe PDF
9.11 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/203037
Citazioni
  • ???jsp.display-item.citation.pmc??? 17
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 26
social impact