Carbon ion therapy (CIT) offers several potential advantages for treating cancers compared with X-ray and proton radiotherapy including increased biological efficacy and more conformal dosimetry. However, CIT potency has not been characterized in primary tumor animal models. Here, we calculate the relative biological effectiveness (RBE) of carbon ions compared to X-rays in an autochthonous mouse model of soft tissue sarcoma. We used Cre/loxP technology to generate primary sarcomas in KrasLSL-G12D/+; p53fl/fl mice. Primary tumors were irradiated with a single fraction of carbon ions (10 Gy), X-rays (20 Gy, 25 Gy, or 30 Gy), or observed as controls. The RBE was calculated by determining the dose of X-rays that resulted in similar time to post-treatment tumor volume quintupling and exponential growth rate as 10 Gy carbon ions. The median tumor volume quintupling time and exponential growth rate of sarcomas treated with 10 Gy carbon ions and 30 Gy X-rays were similar: 27.3 days and 28.1 days, and 0.060 mm3/day and 0.059 mm3/day, respectively. Tumors treated with lower doses of X-rays had faster regrowth. Thus, the RBE of carbon ions in this primary tumor model is 3. When isoeffective treatments of carbon ions and X-rays were compared, we observed significant differences in tumor growth kinetics, proliferative indices, and immune infiltrates. We found that carbon ions were three times as potent as X-rays in this aggressive tumor model and identified unanticipated differences in radiation response that may have clinical implications.

Characterizing the potency and impact of carbon ion therapy in a primary mouse model of soft tissue sarcoma / Brownstein, Jeremy Michael; Wisdom, Amy Jordan; Castle, Katherine D; Mowery, Yvonne M; Guida, Peter M; Lee, Chang-Lung; Tommasino, Francesco; La Tessa, Chiara; Scifoni, Emanuele; Gao, Junheng; Luo, Lixia; Da Silva Campos, Lorraine; Ma, Yan; Williams, Nerissa; Jung, Sin-Ho; Durante, Marco; Kirsch, David G. - In: MOLECULAR CANCER THERAPEUTICS. - ISSN 1535-7163. - 2018:(2018), p. molcanther.0965.2017. [10.1158/1535-7163.MCT-17-0965]

Characterizing the potency and impact of carbon ion therapy in a primary mouse model of soft tissue sarcoma

Tommasino, Francesco;La Tessa, Chiara;Scifoni, Emanuele;Durante, Marco;
2018

Abstract

Carbon ion therapy (CIT) offers several potential advantages for treating cancers compared with X-ray and proton radiotherapy including increased biological efficacy and more conformal dosimetry. However, CIT potency has not been characterized in primary tumor animal models. Here, we calculate the relative biological effectiveness (RBE) of carbon ions compared to X-rays in an autochthonous mouse model of soft tissue sarcoma. We used Cre/loxP technology to generate primary sarcomas in KrasLSL-G12D/+; p53fl/fl mice. Primary tumors were irradiated with a single fraction of carbon ions (10 Gy), X-rays (20 Gy, 25 Gy, or 30 Gy), or observed as controls. The RBE was calculated by determining the dose of X-rays that resulted in similar time to post-treatment tumor volume quintupling and exponential growth rate as 10 Gy carbon ions. The median tumor volume quintupling time and exponential growth rate of sarcomas treated with 10 Gy carbon ions and 30 Gy X-rays were similar: 27.3 days and 28.1 days, and 0.060 mm3/day and 0.059 mm3/day, respectively. Tumors treated with lower doses of X-rays had faster regrowth. Thus, the RBE of carbon ions in this primary tumor model is 3. When isoeffective treatments of carbon ions and X-rays were compared, we observed significant differences in tumor growth kinetics, proliferative indices, and immune infiltrates. We found that carbon ions were three times as potent as X-rays in this aggressive tumor model and identified unanticipated differences in radiation response that may have clinical implications.
Brownstein, Jeremy Michael; Wisdom, Amy Jordan; Castle, Katherine D; Mowery, Yvonne M; Guida, Peter M; Lee, Chang-Lung; Tommasino, Francesco; La Tessa, Chiara; Scifoni, Emanuele; Gao, Junheng; Luo, Lixia; Da Silva Campos, Lorraine; Ma, Yan; Williams, Nerissa; Jung, Sin-Ho; Durante, Marco; Kirsch, David G
Characterizing the potency and impact of carbon ion therapy in a primary mouse model of soft tissue sarcoma / Brownstein, Jeremy Michael; Wisdom, Amy Jordan; Castle, Katherine D; Mowery, Yvonne M; Guida, Peter M; Lee, Chang-Lung; Tommasino, Francesco; La Tessa, Chiara; Scifoni, Emanuele; Gao, Junheng; Luo, Lixia; Da Silva Campos, Lorraine; Ma, Yan; Williams, Nerissa; Jung, Sin-Ho; Durante, Marco; Kirsch, David G. - In: MOLECULAR CANCER THERAPEUTICS. - ISSN 1535-7163. - 2018:(2018), p. molcanther.0965.2017. [10.1158/1535-7163.MCT-17-0965]
File in questo prodotto:
File Dimensione Formato  
Brownstein2018Proofs.pdf

embargo fino al 01/04/2018

Tipologia: Post-print referato (Refereed author’s manuscript)
Licenza: Altra licenza (Other type of license)
Dimensione 1.14 MB
Formato Adobe PDF
1.14 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/202606
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 13
social impact