Genotype assessment of grape regenerants from floral explants

A molecular typing of regenerant vines based on co-dominant simple sequence repeat (SSR) markers was applied for checking recombination events during somatic embryogenesis from floral explants. Twenty-one samples of somatic embryos and plantlets from embryogenic callus of both anthers and ovaries of the V. vinifera cv. Chardonnay, the rootstock Kober 125 AA, and the accession V. rupestris du Lot were randomly chosen from a number of regenerant lines. The genotype at polymorphic VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79 loci was produced and compared with reference patterns. No recombination events were detected in the cells involved in the somatic embryogenesis induction of all the checked samples, since all of them generated the same SSR profile of the grape variety from which explants were isolated.