Silicon oxynitride (SiON) microring resonators areused to develop a biosensor device to detect Aflatoxin M1 (AFM1),a carcinogenic mycotoxin present in milk. Two different surfacefunctionalization strategies are tested to realize an affinity biosens-ing device. Both strategies are based on the formation of a silanesurface layer able to bind either specific DNA-aptamers or anti-gen binding fragments (Fab’). The sensing device consists of anarray of four SiON microring resonators, fiber-coupled to a verti-cal cavity surface emitting laser operating at 850 nm. Detection isrealized with off-chip silicon photodetectors. With both function-alizations, we demonstrate a specific detection of AFM1 in buffer,while the nonspecific detection of Ochratoxin is not occurring. Re-peatability and reproducibility of both functionalization strategiesare discussed.

Aptamer- and Fab’- functionalized microring resonators for Aflatoxin M1 detection / Chalyan, Tatevik; Pasquardini, Laura; Gandolfi, Davide; Guider, Romain; Samusenko, Alina; Zanetti, Manuela; Pucker, Georg; Pederzolli, Cecilia; Pavesi, Lorenzo. - In: IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS. - ISSN 1077-260X. - 23:2(2017), pp. 5900208.1-5900208.8.

Aptamer- and Fab’- functionalized microring resonators for Aflatoxin M1 detection

Tatevik Chalyan;Laura Pasquardini;Davide Gandolfi;Romain Guider;Alina Samusenko;and Lorenzo Pavesi
2017-01-01

Abstract

Silicon oxynitride (SiON) microring resonators areused to develop a biosensor device to detect Aflatoxin M1 (AFM1),a carcinogenic mycotoxin present in milk. Two different surfacefunctionalization strategies are tested to realize an affinity biosens-ing device. Both strategies are based on the formation of a silanesurface layer able to bind either specific DNA-aptamers or anti-gen binding fragments (Fab’). The sensing device consists of anarray of four SiON microring resonators, fiber-coupled to a verti-cal cavity surface emitting laser operating at 850 nm. Detection isrealized with off-chip silicon photodetectors. With both function-alizations, we demonstrate a specific detection of AFM1 in buffer,while the nonspecific detection of Ochratoxin is not occurring. Re-peatability and reproducibility of both functionalization strategiesare discussed.
2
Chalyan, Tatevik; Pasquardini, Laura; Gandolfi, Davide; Guider, Romain; Samusenko, Alina; Zanetti, Manuela; Pucker, Georg; Pederzolli, Cecilia; Pavesi, Lorenzo
Aptamer- and Fab’- functionalized microring resonators for Aflatoxin M1 detection / Chalyan, Tatevik; Pasquardini, Laura; Gandolfi, Davide; Guider, Romain; Samusenko, Alina; Zanetti, Manuela; Pucker, Georg; Pederzolli, Cecilia; Pavesi, Lorenzo. - In: IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS. - ISSN 1077-260X. - 23:2(2017), pp. 5900208.1-5900208.8.
File in questo prodotto:
File Dimensione Formato  
07565645.pdf

Solo gestori archivio

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.2 MB
Formato Adobe PDF
1.2 MB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/192223
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 14
social impact