DD-Carboxypeptidase (DD-CPase) activity of Enterococcus hirae (Streptococcus faecium) ATCC 9790 was extracted from intact bacteria and from the insoluble residue (crude cell wall fraction) of mechanically disrupted bacteria by a brief treatment at pH 10.0 (10 mM glycine-NaOH) at 0 degrees C or by extraction with any of several detergents. Extractions with high salt concentrations failed to remove DD-CPase activity from the crude wall fraction. In contrast to N-acetylmuramoylhydrolase (both muramidase 2 and muramidase 1) activities, DD-CPase activity failed to bind to insoluble cell walls or peptidoglycan matrices. Thus, whereas muramidase 1 and muramidase 2 activities can be considered to be cell wall proteins, the bulk of the data are consistent with the interpretation that the DD-CPase of this species is a membrane protein that is sometimes found in the cell wall fraction, presumably because of hydrophobic interactions with other proteins and cell wall polymers. The binding of [14C]penicillin to penicillin-binding protein 6 (43 kilodaltons) was proportional to DD-CPase activity. Kinetic parameters were also consistent with the presence of only one DD-CPase (penicillin-binding protein 6) in E. hirae.
Properties of cell wall-associated DD-carbossipeptidase of Enterococcus hirae (Streptococcus faecium) ATCC 9790 / Kariyama, R.; Massidda, Orietta; DANEO MOORE, L.; Shockman, G. D.. - In: JOURNAL OF BACTERIOLOGY. - ISSN 0021-9193. - 172:(1990), pp. 3718-3724. [10.1128/jb.172.7.3718-3724.1990]
Titolo: | Properties of cell wall-associated DD-carbossipeptidase of Enterococcus hirae (Streptococcus faecium) ATCC 9790 | |
Autori: | Kariyama, R.; Massidda, Orietta; DANEO MOORE, L.; Shockman, G. D. | |
Autori Unitn: | ||
Titolo del periodico: | JOURNAL OF BACTERIOLOGY | |
Anno di pubblicazione: | 1990 | |
Codice identificativo Scopus: | 2-s2.0-0025315524 | |
Codice identificativo WOS: | WOS:A1990DM73700025 | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1128/jb.172.7.3718-3724.1990 | |
Handle: | http://hdl.handle.net/11572/187556 | |
Citazione: | Properties of cell wall-associated DD-carbossipeptidase of Enterococcus hirae (Streptococcus faecium) ATCC 9790 / Kariyama, R.; Massidda, Orietta; DANEO MOORE, L.; Shockman, G. D.. - In: JOURNAL OF BACTERIOLOGY. - ISSN 0021-9193. - 172:(1990), pp. 3718-3724. [10.1128/jb.172.7.3718-3724.1990] | |
Appare nelle tipologie: | 03.1 Articolo su rivista (Journal article) |
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