Peripheral blood mononuclear cells (PBMCs) were collected from a patient with treatment-refractory Schizophrenia who presented an exceptional clinical response to Clozapine. iPSC lines were established with a non-integrating reprogramming system based on Sendai virus. A footprint-free hiPSC line was characterized to confirm the expression of the main endogenous pluripotency markers and have a regular karyotype. Pluripotency was confirmed by differentiation into cells belonging to the three germ layers. This hiPSC line represents a valuable tool to study the molecular, biochemical and electrophysiological properties of mature neuronal populations belonging to Clozapine responder patients with a severe form of Schizophrenia. © 2016 The Authors. Published by Elsevier B.V.
Establishment of an induced pluripotent stem cell (iPSC) line from a patient with Clozapine-responder Schizophrenia / Marcatili, Matteo; Marsoner, Fabio; D'Agostino, Armando; Karnavas, Thodoris; Bottai, Daniele; Scarone, Silvio; Conti, Luciano. - In: STEM CELL RESEARCH. - ISSN 1873-5061. - ELETTRONICO. - 2016, 17:3(2016), pp. 630-633. [10.1016/j.scr.2016.11.009]
Establishment of an induced pluripotent stem cell (iPSC) line from a patient with Clozapine-responder Schizophrenia
Conti, Luciano
2016
Abstract
Peripheral blood mononuclear cells (PBMCs) were collected from a patient with treatment-refractory Schizophrenia who presented an exceptional clinical response to Clozapine. iPSC lines were established with a non-integrating reprogramming system based on Sendai virus. A footprint-free hiPSC line was characterized to confirm the expression of the main endogenous pluripotency markers and have a regular karyotype. Pluripotency was confirmed by differentiation into cells belonging to the three germ layers. This hiPSC line represents a valuable tool to study the molecular, biochemical and electrophysiological properties of mature neuronal populations belonging to Clozapine responder patients with a severe form of Schizophrenia. © 2016 The Authors. Published by Elsevier B.V.| File | Dimensione | Formato | |
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