Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as “super-refractory”) Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments. © 2016 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with clozapine-resistant Schizophrenia / Marsoner, Fabio; Marcatili, Matteo; Karnavas, Thodoris; Bottai, Daniele; D'Agostino, Armando; Scarone, Silvio; Conti, Luciano. - In: STEM CELL RESEARCH. - ISSN 1873-5061. - ELETTRONICO. - 2016, 17:3(2016), pp. 661-664. [10.1016/j.scr.2016.11.005]
Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with clozapine-resistant Schizophrenia
Conti, Luciano
2016-01-01
Abstract
Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as “super-refractory”) Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments. © 2016 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license| File | Dimensione | Formato | |
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