Spectrally resolved fluorescence lifetime imaging microscopy (λFLIM) has powerful potential for biochemical and medical imaging applications. However, long acquisition times, low spectral resolution and complexity of λFLIM often narrow its use to specialized laboratories. Therefore, we demonstrate here a simple spectral FLIM based on a solid-state detector array providing in-pixel histrogramming and delivering faster acquisition, larger dynamic range, and higher spectral elements than state-of-the-art λFLIM. We successfully apply this novel microscopy system to biochemical and medical imaging demonstrating that solid-state detectors are a key strategic technology to enable complex assays in biomedical laboratories and the clinic. © 2015 Optical Society of America

Fast and simple spectral FLIM for biochemical and medical imaging / Popleteeva, Marina; Haas, Kalina T.; Stoppa, David; Pancheri, Lucio; Gasparini, Leonardo; Kaminski, Clemens F.; Cassidy, Liam D.; Venkitaraman, Ashok R.; Esposito, Alessandro. - In: OPTICS EXPRESS. - ISSN 1094-4087. - ELETTRONICO. - 2015, 23:18(2015), pp. 23511-23525. [10.1364/OE.23.023511]

Fast and simple spectral FLIM for biochemical and medical imaging

Stoppa, David;Pancheri, Lucio;Gasparini, Leonardo;
2015-01-01

Abstract

Spectrally resolved fluorescence lifetime imaging microscopy (λFLIM) has powerful potential for biochemical and medical imaging applications. However, long acquisition times, low spectral resolution and complexity of λFLIM often narrow its use to specialized laboratories. Therefore, we demonstrate here a simple spectral FLIM based on a solid-state detector array providing in-pixel histrogramming and delivering faster acquisition, larger dynamic range, and higher spectral elements than state-of-the-art λFLIM. We successfully apply this novel microscopy system to biochemical and medical imaging demonstrating that solid-state detectors are a key strategic technology to enable complex assays in biomedical laboratories and the clinic. © 2015 Optical Society of America
2015
18
Popleteeva, Marina; Haas, Kalina T.; Stoppa, David; Pancheri, Lucio; Gasparini, Leonardo; Kaminski, Clemens F.; Cassidy, Liam D.; Venkitaraman, Ashok R.; Esposito, Alessandro
Fast and simple spectral FLIM for biochemical and medical imaging / Popleteeva, Marina; Haas, Kalina T.; Stoppa, David; Pancheri, Lucio; Gasparini, Leonardo; Kaminski, Clemens F.; Cassidy, Liam D.; Venkitaraman, Ashok R.; Esposito, Alessandro. - In: OPTICS EXPRESS. - ISSN 1094-4087. - ELETTRONICO. - 2015, 23:18(2015), pp. 23511-23525. [10.1364/OE.23.023511]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11572/159418
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